Mouse Anti-POLR2A Recombinant Antibody (clone 1A6B1)

CAT#: VS7-0425-WR646

This product is a mouse IgG2b antibody that binds to human POLR2A. The antibody 1A6B1 recognizes specific amino acid domain of human POLR2A (AA: 128-258). It can be used for WB and ELISA assays.

Specifications

  • Immunogen
  • Recombinant human POLR2A (AA: 128-258) fragment expressed in E. Coli.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG2b
  • Specificity
  • Human POLR2A
  • Species Reactivity
  • Human
  • Clone
  • 1A6B1
  • Molecule Class
  • RNA Polymerase II Subunit
  • Applications
  • WB, ELISA
  • Conjugate
  • Unconjugated
  • Related Disease
  • Neurodevelopmental disorder with hypotonia and variable intellectual and behavioral abnormalities (NEDHIB)

Product Property

  • Clonality
  • Monoclonal
  • Purity
  • >95% as determined by SDS-PAGE and SEC-HPLC analysis
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • 0.05% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • Western Blot: 1:500 - 1:2000
    Enzyme-linked Immunosorbent Assay: 1:10000

Target

  • Alternative Names
  • POLR2A; Polymerase (RNA) II (DNA Directed) Polypeptide A (220kD); DNA-Directed RNA Polymerase II Subunit A; DNA-Directed RNA Polymerase II Largest Subunit, RNA Polymerase II 220 Kd Subunit; DNA-Directed RNA Polymerase II Subunit RPB1; EC 2.7.7.48; EC 2.7.
  • Long Name
  • RNA polymerase II subunit A
  • Cellular Localization
  • Chromosome; Cytoplasm; DNA-directed RNA polymerase; Nucleus
  • Post Translation Modifications
  • Acetylation; Isopeptide bond; Methylation; Phosphoprotein; Ubl conjugation
  • Function
  • DNA-binding; Hydrolase; Nucleotidyltransferase; RNA-directed RNA polymerase; Transferase
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

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Isotype Control

Secondary Antibody

Reconstitution Buffer

Recommended Dilution Buffer

See other products for "POLR2A"

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MOR-2782 Hi-Affi™ Recombinant Rabbit Anti-POLR2A Monoclonal Antibody (DS2782AB) WB, IP, IHC-P, ICC, IF IgG
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VS-0424-XY213 AbPlus™ Anti-POLR2A Magnetic Beads (JM11-51) IP, Protein Purification
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