Mouse Anti-POLR2A Recombinant Antibody (clone 1B1D8) (CAT#: VS7-0425-WR647)
This product is a mouse IgG2b antibody that binds to human POLR2A. The antibody 1B1D8 recognizes specific amino acid domain of human POLR2A (AA: 128-258). It can be used for FC assay.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Specifications
- Immunogen
- Recombinant human POLR2A (AA: 128-258) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human POLR2A
- Species Reactivity
- Human
- Clone
- 1B1D8
- Molecule Class
- RNA Polymerase II Subunit
- Applications
- FC
- Conjugate
- Unconjugated
- Related Disease
- Neurodevelopmental disorder with hypotonia and variable intellectual and behavioral abnormalities (NEDHIB)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- POLR2A; Polymerase (RNA) II (DNA Directed) Polypeptide A (220kD); DNA-Directed RNA Polymerase II Subunit A; DNA-Directed RNA Polymerase II Largest Subunit, RNA Polymerase II 220 Kd Subunit; DNA-Directed RNA Polymerase II Subunit RPB1; EC 2.7.7.48; EC 2.7.
- Gene ID
- 5430
- UniProt ID
- P24928
- Long Name
- RNA polymerase II subunit A
- Cellular Localization
- Chromosome; Cytoplasm; DNA-directed RNA polymerase; Nucleus
- Post Translation Modifications
- Acetylation; Isopeptide bond; Methylation; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_000928.1
- Function
- DNA-binding; Hydrolase; Nucleotidyltransferase; RNA-directed RNA polymerase; Transferase
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2422z | Mouse Anti-POLR2A Recombinant Antibody (clone 23G8) | WB, ELISA, FC, ICC, IF, IP | Mouse IgG1 |
| MRO-2362-CN | Recombinant Rabbit Anti-POLR2A (phosphorylated Ser2) Monoclonal Antibody (JM11-51) | WB, IP, IF, IHC, FC | Rabbit IgG |
| MRO-2363-CN | Recombinant Rabbit Anti-POLR2A (phosphorylated Ser5) Monoclonal Antibody (JM51-21) | WB, IP, IF, IHC, FC | Rabbit IgG |
| VS3-CJ812 | Mouse Anti-POLR2A Recombinant Antibody (clone 1357CT288.1.37) | ELISA, WB | Mouse IgG1, κ |
| VS7-0425-WR646 | Mouse Anti-POLR2A Recombinant Antibody (clone 1A6B1) | WB, ELISA | Mouse IgG2b |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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