Mouse Anti-POLR2A Recombinant Antibody (clone 1B1D8)
CAT#: VS7-0425-WR647
This product is a mouse IgG2b antibody that binds to human POLR2A. The antibody 1B1D8 recognizes specific amino acid domain of human POLR2A (AA: 128-258). It can be used for FC assay.
Specifications
- Immunogen
- Recombinant human POLR2A (AA: 128-258) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human POLR2A
- Species Reactivity
- Human
- Clone
- 1B1D8
- Molecule Class
- RNA Polymerase II Subunit
- Applications
- FC
- Conjugate
- Unconjugated
- Related Disease
- Neurodevelopmental disorder with hypotonia and variable intellectual and behavioral abnormalities (NEDHIB)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- POLR2A; Polymerase (RNA) II (DNA Directed) Polypeptide A (220kD); DNA-Directed RNA Polymerase II Subunit A; DNA-Directed RNA Polymerase II Largest Subunit, RNA Polymerase II 220 Kd Subunit; DNA-Directed RNA Polymerase II Subunit RPB1; EC 2.7.7.48; EC 2.7.
- Gene ID
- 5430
- UniProt ID
- P24928
- Long Name
- RNA polymerase II subunit A
- Cellular Localization
- Chromosome; Cytoplasm; DNA-directed RNA polymerase; Nucleus
- Post Translation Modifications
- Acetylation; Isopeptide bond; Methylation; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_000928.1
- Function
- DNA-binding; Hydrolase; Nucleotidyltransferase; RNA-directed RNA polymerase; Transferase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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CAT | Product Name | Application | Type |
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CAT | Product Name | Application | Type |
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CAT | Product Name | Application | Type |
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CAT | Product Name | Application | Type |
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