Anti-PPM1A Immunohistochemistry Kit
CAT#: VS-0325-XY1714
Our PPM1A IHC Kit contains a full set of reagents, covering every aspect of immunohistochemical procedures. It is designed with user - friendliness in mind, featuring a simple protocol that even beginners can follow without difficulty. This kit is highly adaptable as it can be applied to both paraffin - embedded and frozen tissue sections, allowing for accurate PPM1A detection in a broad range of histological samples for different research undertakings.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29209/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29209/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Glial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29209/117050_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29209/117050_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29209/117050_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29209/117050_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29209/117050_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29209/117050_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100614-PPM1A/subcellular
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Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human, Mouse, Rat
- Target
- PPM1A
- Primary Antibody
- Rabbit Anti-PPM1A Antibody
- Secondary Antibody
- Goat anti-Rabbit Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| MOB-1479CT | Recombinant Mouse anti-Human PPM1A Monoclonal antibody (q7d8) | ELISA, IHC-P, WB | |
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| VS3-QX902 | Mouse Anti-PPM1A Recombinant Antibody (clone 1D2-E6-F2) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
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| CAT | Product Name | Application | Type |
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| MOR-2811 | Hi-Affi™ Recombinant Rabbit Anti-PPM1A Monoclonal Antibody (DS2811AB) | WB, IP, IHC-P, ICC, FC | IgG |
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| VS-0724-YC1092 | AbPlus™ Anti-PPM1A Magnetic Beads (VS-0724-YC1092) | IP, Protein Purification | |
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| CAT | Product Name | Application | Type |
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| VS-1024-XY402 | Mouse Anti-NHP PPM1A Recombinant Antibody (clone 7F12) | WB, IF, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| VS-0525-XY5609 | Anti-Human PPM1A Immunohistochemistry Kit | IHC |
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