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    Mouse Anti-NHP PPM1A Recombinant Antibody (clone 7F12) (CAT#: VS-1024-XY402)

    This finely tuned mouse monoclonal antibody is crafted to target PPM1A, exhibiting substantial reactivity with human, mouse, non-human primate, and rat samples. Utilizing a purified recombinant protein fragment as the immunogen, it is highly versatile for applications including Western blotting, immunofluorescence, flow cytometry, and ELISA. Researchers can employ this antibody to investigate the pivotal roles of PPM1A in cellular signaling and stress responses, thereby advancing our understanding of its contributions to physiological regulation and potential implications in various pathological conditions across multiple species.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/29209/if_selected.jpg
    Figure 2 IHC staining of human bone marrow
    Figure 2 IHC staining of human bone marrow
    (Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/29209/ihc_selected.jpg
    Normal Tissue
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Endothelial cells
    Staining: Low
    Intensity: Moderate
    Quantity: <25%
    Location: Cytoplasmic/membranous
    Glial cells
    Staining: Low
    Intensity: Weak
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Neuronal cells
    Staining: Low
    Intensity: Weak
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Neuropil
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/29209/117050_B_8_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endothelial cells
    Staining: Low
    Intensity: Weak
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Glandular cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Peripheral nerve/ganglion
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/29209/117050_A_8_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Cholangiocytes
    Staining: Low
    Intensity: Weak
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Hepatocytes
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/29209/117050_A_8_4.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in glomeruli
    Staining: Not detected
    Intensity: Weak
    Quantity: <25%
    Location: Cytoplasmic/membranous
    Cells in tubules
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/29209/117050_A_8_5.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Cells in seminiferous ducts
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Leydig cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/29209/117050_A_4_6.jpg
    Figure 8 Lymph node
    Figure 8 Lymph node
    (Germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Non-germinal center cells
    Staining: Medium
    Intensity: Strong
    Quantity: <25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/29209/117050_A_8_8.jpg
    RNA Expression
    Figure 9 RNA cell line category: Low cell line specificity
    Figure 9 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100614-PPM1A/subcellular

    Specifications

    • Immunogen
    • Purified recombinant fragment
    • Host Species
    • Mouse
    • Type
    • Mouse IgG1
    • Specificity
    • PPM1A
    • Species Reactivity
    • Human, Mouse, Non-human primate, Rat
    • Clone
    • 7F12
    • Applications
    • WB, IF, FC, ELISA

    Product Property

    • Purification
    • Protein A purified
    • Purity
    • >95%, determined by SDS-PAGE
    • Format
    • Liquid
    • Buffer
    • PBS, pH 7.4
    • Storage
    • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

    Applications

    • Application Notes
    • WB: 1:500-1:1000, IF: 5 µg/mL, FC: 1:200-1:400, ELISA: 1:8000

    Target

    • Alternative Names
    • PP2CA; PP2Calpha; PP2C-ALPHA

    Recommended Products

    Recommended Dilution Buffer

    Isotype Control

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "PPM1A"

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    CAT Product Name Application Type
    MOB-1479CT Recombinant Mouse anti-Human PPM1A Monoclonal antibody (q7d8) ELISA, IHC-P, WB
    MRO-2128-CN Rabbit Anti-PPM1A Polyclonal Antibody (MRO-2128-CN) WB, IF, IHC, FC Rabbit IgG
    VS3-QX902 Mouse Anti-PPM1A Recombinant Antibody (clone 1D2-E6-F2) WB, IHC, ICC, FC, ELISA Mouse IgG1
    VS3-WK174 Mouse Anti-PPM1A Recombinant Antibody (clone H6-F12) WB, ELISA Mouse IgG1
    VS7-0425-WR658 Mouse Anti-PPM1A Recombinant Antibody (clone 7F12H6) WB, FC Mouse IgG1

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    For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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