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    Mouse Anti-PPM1A Recombinant Antibody (clone 7F12H6) (CAT#: VS7-0425-WR658)

    This product is a mouse IgG1 antibody that binds to human PPM1A. The antibody 7F12H6 recognizes specific amino acid domain of human PPM1A (AA: 202-382). It can be used for WB and FC assays. It cross reacts with monkey.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/29209/if_selected.jpg
    Figure 2 IHC staining of human bone marrow
    Figure 2 IHC staining of human bone marrow
    (Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/29209/ihc_selected.jpg
    Normal Tissue
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Endothelial cells
    Staining: Low
    Intensity: Moderate
    Quantity: <25%
    Location: Cytoplasmic/membranous
    Glial cells
    Staining: Low
    Intensity: Weak
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Neuronal cells
    Staining: Low
    Intensity: Weak
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Neuropil
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/29209/117050_B_8_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endothelial cells
    Staining: Low
    Intensity: Weak
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Glandular cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Peripheral nerve/ganglion
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/29209/117050_A_8_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Cholangiocytes
    Staining: Low
    Intensity: Weak
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Hepatocytes
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/29209/117050_A_8_4.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in glomeruli
    Staining: Not detected
    Intensity: Weak
    Quantity: <25%
    Location: Cytoplasmic/membranous
    Cells in tubules
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/29209/117050_A_8_5.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Cells in seminiferous ducts
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Leydig cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/29209/117050_A_4_6.jpg
    Figure 8 Lymph node
    Figure 8 Lymph node
    (Germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Non-germinal center cells
    Staining: Medium
    Intensity: Strong
    Quantity: <25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/29209/117050_A_8_8.jpg
    RNA Expression
    Figure 9 RNA cell line category: Low cell line specificity
    Figure 9 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100614-PPM1A/subcellular

    Specifications

    • Immunogen
    • Recombinant human PPM1A (AA: 202-382) fragment expressed in E. Coli.
    • Host Species
    • Mouse
    • Type
    • Mouse IgG1
    • Specificity
    • Human PPM1A
    • Species Reactivity
    • Human, Monkey
    • Clone
    • 7F12H6
    • Molecule Class
    • Serine/Threonine Protein Phosphatase
    • Applications
    • WB, FC
    • Conjugate
    • Unconjugated

    Product Property

    • Clonality
    • Monoclonal
    • Purity
    • >95% as determined by SDS-PAGE and SEC-HPLC analysis
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • PBS
    • Preservative
    • 0.05% sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • Western Blot: 1:500 - 1:2000
      Flow Cytometry: 1:200 - 1:400
      Enzyme-linked Immunosorbent Assay: 1:10000

    Target

    • Alternative Names
    • PP2CA; PP2Calpha; PP2C-ALPHA
    • Long Name
    • Protein phosphatase, Mg2+/Mn2+ dependent 1A
    • Cellular Localization
    • Nucleus
    • Post Translation Modifications
    • Phosphoprotein
    • Function
    • Activator; DNA-binding

    Recommended Products

    Isotype Control

    Secondary Antibody

    Reconstitution Buffer

    Recommended Dilution Buffer

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "PPM1A"

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    CAT Product Name Application Type
    MOB-1479CT Recombinant Mouse anti-Human PPM1A Monoclonal antibody (q7d8) ELISA, IHC-P, WB
    MRO-2128-CN Rabbit Anti-PPM1A Polyclonal Antibody (MRO-2128-CN) WB, IF, IHC, FC Rabbit IgG
    VS3-QX902 Mouse Anti-PPM1A Recombinant Antibody (clone 1D2-E6-F2) WB, IHC, ICC, FC, ELISA Mouse IgG1
    VS3-WK174 Mouse Anti-PPM1A Recombinant Antibody (clone H6-F12) WB, ELISA Mouse IgG1

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