Rabbit Anti-PPP1CB Recombinant Antibody (clone R09-6B7)
CAT#: VS3-FY1844
The R09-6B7 recombinant monoclonal antibody binds to human PPP1CB. It cross-reacts with mouse, rat. The antibody is recommended for detection of PPP1CB protein by WB, IHC-F, IHC-P, ICC/IF, IP. The recommended dilution ratio is as follows: western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). The PP1C beta antibody is guaranteed for western blot analysis of PPP1CB in K562, rat brain, C6, 3T3, Hela lysates.
Tested Data
WB
Figure 1 Anti-PPP1CB Antibody (VS3-FY1844) in WB.
The PPP1CB expression in K562, rat Brain, C6, 3T3, Hela lysates were detected using the anti-PP1C beta by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human PPP1CB
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PPP1CB
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R09-6B7
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 37 kDa, while the observed molecular weight stands at 37 kDa.
- Related Disease
- Noonan Syndrome-Like Disorder With Loose Anagen Hair 2 and Noonan Syndrome-Like Disorder With Loose Anagen Hair
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- MP; PP1B; PP1c; NSLH2; PP-1B; PPP1CD; PP1beta; PPP1beta; HEL-S-80p
- Gene ID
- 5500
- UniProt ID
- P62140
- Sequence Similarities
- Belongs to the PPP phosphatase family. PP-1 subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys25 and Lys303 (NX_P62140 [NX_P62140-1])
Modification sites at PhosphoSitePlus (P62140)
Glycosylation from GlyGen (P62140) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_002700.1; NP_996759.1
- Function
- Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PPP1CB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2271CT | Recombinant Mouse anti-Human PPP1CB Monoclonal antibody (EDT) | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2815 | Hi-Affi™ Recombinant Rabbit Anti-PPP1CB Monoclonal Antibody (DS2815AB) | WB, IP, FC, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK365 | Rabbit Anti-PPP1CB Recombinant Antibody (clone JE45-08) | WB, IP, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-XY1290 | Mouse Anti-PPP1CB Recombinant Antibody (clone 8A7C7) | IHC, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS7-0425-WR663 | Mouse Anti-PPP1CB Recombinant Antibody (clone 6G10C1) | WB, IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS7-0425-WR664 | Mouse Anti-PPP1CB Recombinant Antibody (VS7-0425-WR664) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1718 | Anti-PPP1CB Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5619 | Anti-Mouse PPP1CB Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5618 | Anti-Human PPP1CB Immunohistochemistry Kit | IHC |
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