Mouse Anti-PPP2CA Recombinant Antibody (VS3-CJ36)
CAT#: VS3-CJ36
This product is a mouse antibody that recognizes PPP2CA.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113575-PPP2CA/subcellular
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Specifications
- Immunogen
- Recombinant full length of human PPP2CA
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PPP2CA
- Species Reactivity
- Human
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/mL (lot specific)
- Buffer
- 50% Glycerol, 1% BSA, PBS, pH7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).
Target
- Alternative Names
- NEDLBA; PP2Ac; PP2CA; PP2Calpha; RP-C
- Gene ID
- 5515
- UniProt ID
- P67775
- Sequence Similarities
- Belongs to the PPP phosphatase family. PP-1 subfamily.
- Cellular Localization
- Centromere, Chromosome, Cytoplasm, Cytoskeleton, Nucleus
- Post Translation Modifications
- Reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
Polyubiquitinated, leading to its degradation by the proteasome.
- Protein Refseq
- NP_002706.1
- Function
- PP2A is the major phosphatase for microtubule-associated proteins (MAPs) (PubMed:22613722).
PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase (PubMed:22613722).
Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity).
Can dephosphorylate SV40 large T antigen and p53/TP53 (PubMed:17245430).
Activates RAF1 by dephosphorylating it at 'Ser-259' (PubMed:10801873).
Mediates dephosphorylation of WEE1, preventing its ubiquitin-mediated proteolysis, increasing WEE1 protein levels, and promoting the G2/M checkpoint (PubMed:33108758).
Mediates dephosphorylation of MYC; promoting its ubiquitin-mediated proteolysis: interaction with AMBRA1 enhances interaction between PPP2CA and MYC (PubMed:25438055).
Mediates dephosphorylation of FOXO3; promoting its stabilization: interaction with AMBRA1 enhances interaction between PPP2CA and FOXO3 (PubMed:30513302).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "PPP2CA"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC159 | PE-A*02:01/Human Ser/Thr protein phosphatase 2A (SLLPAIVEL) MHC Tetramer | FCM | |
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| CAT | Product Name | Application | Type |
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| MOR-2825 | Hi-Affi™ Recombinant Rabbit Anti-PPP2CA Monoclonal Antibody (DS2825AB) | WB, FC, IP, IHC | IgG |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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