Mouse Anti-PRKAB2 Recombinant Antibody (clone 8F5B2) (CAT#: VS7-0425-WR686)

• Immunogen
• Recombinant human PRKAB2 (AA: 1-120) fragment expressed in E. Coli.

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human PRKAB2

• Species Reactivity
• Human

• Clone
• 8F5B2

• Molecule Class
• Ser/Thr Protein Kinase Family

• Applications
• IHC, ICC, FC

• Conjugate
• Unconjugated

• Clonality
• Monoclonal

• Purity
• >95% as determined by SDS-PAGE and SEC-HPLC analysis

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS

• Preservative
• 0.05% sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• Western Blot: 1:500 - 1:2000
  Immunohistochemistry-Paraffin: 1:200 - 1:1000
  Immunocytochemistry: 1:200 - 1:1000
  Flow Cytometry: 1:200 - 1:400
  Enzyme-linked Immunosorbent Assay: 1:10000

• Alternative Names
• PRKAB2; protein kinase, AMP-activated, beta 2 non-catalytic subunit; 5-AMP-activated protein kinase subunit beta-2; AMPK beta 2; 5 AMP activated protein kinase beta 2 subunit; 5 AMP activated protein kinase subunit beta 2; AMP activated protein kinase beta 2 non catalytic subunit; AMPK beta 2; AMPK beta 2 chain; AMPK subunit beta 2; PRKAB 2; Protein kinase AMP activated beta 2 non catalytic subunit; AMPK beta-2 chain; AMPK subunit beta-2; 5-AMP-activated protein kinase, beta-2 subunit;

• Gene ID
• 5565

• UniProt ID
• O43741

• Long Name
• Protein Kinase, AMP-Activated, Beta 2 Non-Catalytic Subunit

• Cellular Localization
• cytoplasm; cytosol; nucleoplasm; nucleus

• Post Translation Modifications
• Phosphoprotein

• Protein Refseq
• NP_005390.1

• Function
• Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

Secondary Antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Reconstitution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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