Mouse Anti-PRKAG1 Recombinant Antibody (clone 3G12) (CAT#: VS3-WK1596)

• Immunogen
• Synthesized peptide derived from human AMPK γ1

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human PRKAG1

• Species Reactivity
• Human, Mouse, Rat

• Clone
• 3G12

• Applications
• IHC-P, WB

• Conjugate
• Unconjugated

• Clonality
• Monoclonal

• Purification
• Protein G affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Aggregation
• < 5%

• Format
• Liquid

• Concentration
• 1 mg/mL

• Buffer
• PBS, 0.5% BSA, 50% Glycerol

• Preservative
• 0.02% Sodium Azide

• Storage
• Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• IHC-P: 1/50-1/200
  WB: 1/1000-1/2000

• Alternative Names
• AMPKG

• Gene ID
• 5571

• UniProt ID
• P54619

• Long Name
• Protein kinase AMP-activated non-catalytic subunit gamma 1

• Sequence Similarities
• Belongs to the 5'-AMP-activated protein kinase gamma subunit family.

• Cellular Localization
• Cytosol, Nucleoplasm, Nucleus

• Post Translation Modifications
• Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK.

• Protein Refseq
• NP_001193638.1; NP_001193639.1; NP_002724.1

• Function
• AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin.

Secondary Antibody

• 2RD-A1514-YJ
• Sheep Anti-Mouse IgG-heavy and light chain Secondary antibody

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

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