Recombinant Rabbit Anti-PRKAG1 Antibody (clone 5E5) (CAT#: VS4-CJ90)

• Immunogen
• A synthesized peptide derived from human PRKAG1

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human PRKAG1

• Species Reactivity
• Human

• Clone
• 5E5

• Applications
• ELISA, IHC

• Conjugate
• Unconjugated

• Purification
• Affinity chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 150 mM NaCl, PBS, pH 7.4.

• Preservative
• 0.02% Sodium Azide

• Storage
• Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

• Application Notes
• This antibody has been tested for use in ELISA, Immunohistochemistry (1:50-1:200).

• Alternative Names
• AMPKG

• Gene ID
• 5571

• UniProt ID
• P54619

• Long Name
• Protein kinase AMP-activated non-catalytic subunit gamma 1

• Sequence Similarities
• Belongs to the 5'-AMP-activated protein kinase gamma subunit family.

• Cellular Localization
• Cytosol, Nucleoplasm, Nucleus

• Post Translation Modifications
• Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK.

• Protein Refseq
• NP_001193638.1; NP_001193639.1; NP_002724.1

• Function
• AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS