Mouse Anti-PRKAG1 Recombinant Antibody (clone 5H5)
CAT#: VS3-WK1593
This antibody is a mouse recombinant antibody against human PRKAG1, which is a regulatory subunit of the AMP-activated protein kinase (AMPK). The clone 5H5 can be used in WB analysis at a dilution of 1/1000-1/2000 and IHC analysis at a dilution of 1/50-1/200.
Specifications
- Immunogen
- Synthesized peptide derived from human AMPK γ1
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PRKAG1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 5H5
- Applications
- IHC-P, WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 0.5% BSA, 50% Glycerol
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- IHC-P: 1/50-1/200
WB: 1/1000-1/2000
Target
- Alternative Names
- AMPKG
- Gene ID
- 5571
- UniProt ID
- P54619
- Long Name
- Protein kinase AMP-activated non-catalytic subunit gamma 1
- Sequence Similarities
- Belongs to the 5'-AMP-activated protein kinase gamma subunit family.
- Cellular Localization
- Cytosol, Nucleoplasm, Nucleus
- Post Translation Modifications
- Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK.
- Protein Refseq
- NP_001193638.1; NP_001193639.1; NP_002724.1
- Function
- AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
Isotype Control
- CAT
- Product Name
See other products for "PRKAG1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2849 | Hi-Affi™ Recombinant Rabbit Anti-PRKAG1 Monoclonal Antibody (DS2849AB) | FC, ICC, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-XY1302 | Mouse Anti-PRKAG1 Recombinant Antibody (clone 4A1G9) | IHC | Mouse IgG1 |
| VS4-CJ90 | Recombinant Rabbit Anti-PRKAG1 Antibody (clone 5E5) | ELISA, IHC | Rabbit IgG |
| VS3-WK1592 | Mouse Anti-PRKAG1 Recombinant Antibody (clone 5A1) | IHC | Mouse IgG |
| VS3-WK1596 | Mouse Anti-PRKAG1 Recombinant Antibody (clone 3G12) | IHC-P, WB | Mouse IgG |
| VS13-YC930 | Rabbit Anti-PRKAG1 Recombinant Antibody (VS13-YC930) | WB, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1746 | Anti-PRKAG1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5676 | Anti-Human PRKAG1 Immunohistochemistry Kit | IHC |
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