Rabbit Anti-PSEN1 Recombinant Antibody (clone R01-3J6)
CAT#: VS3-XY3477
This antibody is a recombinant rabbit antibody against PSEN1 (Presenilin 1). The antibody specifically recognizes human PSEN1. It also has cross-reactivity against mouse and rat PSEN1. It has been reported for use in Western Blot and Flow cytometry. The recommended dilutions of Western Blot and Flow cytometry is 1/500-1/1000 and 1/50-1/100, respectively.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus & cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67496/1462_C6_5_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity with a granular pattern in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30760/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30760/83531_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30760/83531_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30760/83531_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30760/83531_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30760/83531_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30760/83531_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000080815-PSEN1/subcellular
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PSEN1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-3J6
- Applications
- WB, FC
- Related Disease
- Alzheimer Disease 3; Cardiomyopathy, Dilated, 1U
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
FC: 1/50-1/100
Target
- Alternative Names
- AD3; FAD; PS1; PS-1; S182; PSNL1; ACNINV3
- Gene ID
- 5663
- UniProt ID
- A0A024R6A3
- Long Name
- Presenilin 1
- Cellular Localization
- Endoplasmic reticulum, Endoplasmic reticulum membrane
- Post Translation Modifications
- After endoproteolysis, the C-terminal fragment (CTF) is phosphorylated on serine residues by PKA and/or PKC.
Phosphorylation on Ser-346 inhibits endoproteolysis.
(P49768-PSN1_HUMAN)
Modification sites at PhosphoSitePlus (P49768)
Modification sites at neXtProt (NX_P49768)
- Protein Refseq
- XP_005267923.1; XP_005267921.1
- Function
- Catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed:15274632, 10545183, 10593990, 10206644, 10899933, 10811883, 12679784, 12740439, 25043039, 26280335, 30598546, 30630874, 28269784, 20460383).
Requires the presence of the other members of the gamma-secretase complex for protease activity (PubMed:15274632, 25043039, 26280335, 30598546, 30630874).
Plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PSEN1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1791z | Mouse Anti-PSEN1 Recombinant Antibody (clone 35G2) | WB, ELISA, IF, IP | Mouse IgG1, κ |
| MOB-1686CT | Recombinant Mouse anti-Human PSEN1 Monoclonal antibody (45-5D4) | IP, WB | |
| VS3-FY1204 | Recombinant Rabbit Anti-PSEN1 Antibody (clone R05-4D4) | WB, ICC, IF, IP | Rabbit IgG |
| VS3-WK1980 | Rabbit Anti-PSEN1 (pS310) Recombinant Antibody (clone R07-6E3) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0001MZ | Recombinant Chicken Anti-PSEN1 IgY | N/A | Chicken antibody |
| BRD-1139MZ | Chicken Anti-Presenilin 1 Polyclonal IgY | WB, ELISA | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2883 | Hi-Affi™ Recombinant Rabbit Anti-PSEN1 Monoclonal Antibody (DS2883AB) | WB, IP, ICC, FC, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1278 | AbPlus™ Anti-PSEN1 Magnetic Beads (VS-0724-YC1278) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY407 | Mouse Anti-NHP PSEN1 Recombinant Antibody (clone APS 18) | WB, IHC, ICC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1771 | Anti-PSEN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5764 | Anti-Mouse PSEN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5763 | Anti-Human PSEN1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC91 | Recombinant Anti-PSEN1 Vesicular Antibody, EV Displayed (VS-0425-YC91) | ELISA, FC, Cell-uptake |
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