Rabbit Anti-PSENEN Recombinant Antibody (clone R08-5F0)
CAT#: VS3-FY2060
The R08-5F0 is a rabbit recombinant monoclonal antibody that exhibits specific binding to human PSENEN. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. The R08-5F0 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200).
Tested Data
Gene Expression
ICC
Figure 1 Anti-PEN2 Antibody (VS3-FY2060) in ICC.
Immunocytochemistry staining of PEN2 (green) in Hela using the anti-PEN2 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
WB
Figure 2 Anti-PEN2 Antibody (VS3-FY2060) in WB.
The PEN2 expression in Raw264.7, mouse brain lysates were detected using the anti-PEN2 by western blot assay.
❮
❯
Normal Tissue
Figure 1 Cerebral cortex
Neuronal cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47435/124431_B_9_5.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47435/124431_A_8_3.jpg
Normal Tissue
Figure 3 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47435/124431_A_7_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47435/124431_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47435/124431_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47435/124431_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000205155-PSENEN/subcellular
❮
❯
Specifications
- Immunogen
- Synthetic peptide of human PEN2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PSENEN
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-5F0
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 12 kDa, while the observed molecular weight stands at 12 kDa.
- Related Disease
- Acne Inversa, Familial, 2, With Or Without Dowling-Degos Disease and Dowling-Degos Disease
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- PSENEN; MSTP064; PEN-2; PEN2; MDS033; presenilin enhancer gamma-secretase subunit; ACNINV2; presenilin enhancer; gamma-secretase subunit
- Gene ID
- 55851
- UniProt ID
- Q9NZ42
- Sequence Similarities
- Belongs to the PEN-2 family.
- Cellular Localization
- Cell membrane, Endoplasmic reticulum, Golgi apparatus, Membrane
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q9NZ42)
Modification sites at neXtProt (NX_Q9NZ42)
- Protein Refseq
- NP_001268461.1; NP_758844.1
- Function
- The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels (Probable). PSENEN modulates both endoproteolysis of presenilin and gamma-secretase activity.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY2060. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PSENEN"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0971MZ | Recombinant Mouse Anti-Human PSENEN Antibody | WB | Mouse antibody |
| MRO-1203-CN | Recombinant Rabbit Anti-PSENEN Monoclonal Antibody (JE41-28) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-WK354 | Rabbit Anti-PSENEN Recombinant Antibody (clone JE41-28) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2885 | Hi-Affi™ Recombinant Rabbit Anti-PSENEN Monoclonal Antibody (DS2885AB) | WB, IHC-P, ICC, IF, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5767 | Anti-PSENEN Immunohistochemistry Kit | IHC | |
| VS-0525-XY5768 | Anti-Mouse PSENEN Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, IP, WB, IHC, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, Vaccine, FuncS
Application: ELISA, FuncS, RIA, IP, IF, IHC, IA, Block
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
