Recombinant Human Anti-PSIP1 Single Domain Antibody (PABC-550)
CAT#: PABC-550
This product is a recombinant Human antibody that can recognize PSIP1. VH59 inhibits HIV replication of CD4 T cells by competing HIV integrase binding to LEDGF IBD. VH59 binds to LEDGF IBD at an almost identical position as the integrase.
Published Data
Gene Expression
Neut
Figure 1 Human Anti-PSIP1 Single Domain Antibody (VH59) in Neut.
VH59 disrupts the interaction of LEGDF IBD with HIV IN in Jurkat T cell inhibiting the production of viral capsid protein p24.
Bao, L. , Hannon, C. , Cruz-Mignoni, A. , Ptchelkine, D. , Sun, M. Y. , & Miller, A. , et al. Intracellular immunization against hiv infection with an intracellular antibody that mimics hiv integrase binding to the cellular ledgf protein. Scientific Reports.
Luciferase
Figure 2 Human Anti-PSIP1 Single Domain Antibody (VH59) in Luciferase.
Interaction of LEGDF IBD and anti-IBD VH59 in mammalian cells. The interaction of the LEGDF IBD with the iDab VH59 was tested by mammalian two-hybrid analysis using a luciferase reporter system in CHO cells. LEDGF IBD or LMO2 control baits were expressed as fusions with GAL4 DBD (pM1 vector) and various VH iDAbs or HIV IN were expressed as prey fusions with the VP16 activation domain (pEF-VP16 vector). Luciferase reporter activity in transfected CHO cells. Forty-eight hours after transfection, cells were lysed and luciferase activities were measured. Firefly/Renilla luciferase activity was calculated for each transformation pair.
Bao, L. , Hannon, C. , Cruz-Mignoni, A. , Ptchelkine, D. , Sun, M. Y. , & Miller, A. , et al. Intracellular immunization against hiv infection with an intracellular antibody that mimics hiv integrase binding to the cellular ledgf protein. Scientific Reports.
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Subcellular Location and Protein Expression
Figure 1 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong nuclear as well as cytoplasmic positivity in neuronal and glial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/178_B4_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/247_B4_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/177_B4_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/46099_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Endocrine cells
Staining:High
Intensity: Strong
Endothelial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Enterocytes
Staining:High
Intensity: Strong
Quantity:>75%
Fibroblasts
Staining:High
Intensity: Strong
Goblet cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Mucosal lymphoid cells
Staining:High
Intensity: Strong
Quantity:>75%
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/46212_A_8_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/46212_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/46212_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Peritubular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/46212_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/46212_A_7_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (HEL)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164985-PSIP1
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Specifications
- Host Species
- Human
- Type
- Human VHH
- Specificity
- Human PSIP1
- Species Reactivity
- Human
- Clone
- PABC-550
- Applications
- ELISA, WB, Inhib
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Lot Number:
See other products for "PSIP1"
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| DrMAB-487 | Recombinant Human Anti-Human PSIP1 Antibody, clone D484 | ELISA, IP-MS | IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS-0325-XY1773 | Anti-PSIP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5771 | Anti-Mouse PSIP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5770 | Anti-Human PSIP1 Immunohistochemistry Kit | IHC |
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