Mouse Anti-NHP PSIP1 Recombinant Antibody (clone 6E4)

Name: Mouse Anti-NHP PSIP1 Recombinant Antibody (clone 6E4)
SKU: VS-1024-XY415
Rating: 5 (1 reviews)

CAT#: VS-1024-XY415

Targeting PSIP1, this mouse monoclonal antibody showcases reactivity with human, mouse, non-human primate, and rat tissues. The immunogen is a purified recombinant fragment of human PSIP1, ensuring precise binding and high specificity. Ideal for applications in Western blot (WB), immunohistochemistry (IHC), and ELISA, this antibody is an essential tool for studying PSIP1 expression and function across various research contexts, providing reliable results and enhancing experimental accuracy.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IHC staining of human cerebral cortex

Immunohistochemical staining of human cerebral cortex shows strong nuclear as well as cytoplasmic positivity in neuronal and glial cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/178_B4_1_red_green.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/247_B4_1_red_green.jpg

Subcellular Location and Protein Expression Figure 4 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/177_B4_1_red_green.jpg

Normal Tissue Figure 5 Cerebral cortex

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/46099_B_9_5.jpg

Normal Tissue Figure 6 Colon

Endocrine cells
Staining:High
Intensity: Strong
Endothelial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Enterocytes
Staining:High
Intensity: Strong
Quantity:>75%
Fibroblasts
Staining:High
Intensity: Strong
Goblet cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Mucosal lymphoid cells
Staining:High
Intensity: Strong
Quantity:>75%
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/46212_A_8_3.jpg

Normal Tissue Figure 7 Liver

Cholangiocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/46212_A_9_4.jpg

Normal Tissue Figure 8 Kidney

Cells in glomeruli
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/46212_A_8_5.jpg

Normal Tissue Figure 9 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Peritubular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/46212_A_6_6.jpg

Normal Tissue Figure 10 Lymph node

Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19697/46212_A_7_8.jpg

RNA Expression Figure 11 RNA cell line category: Cell line enhanced (HEL)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164985-PSIP1

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Specifications

Immunogen
Purified recombinant fragment of human PSIP1

Host Species
Mouse

Type
Mouse IgG1

Specificity
PSIP1

Species Reactivity
Human, Mouse, Non-human primate, Rat

Clone
6E4

Applications
WB, IHC, ELISA

Product Property

Purification
Protein A purified

Purity
>95%, determined by SDS-PAGE

Format
Liquid

Buffer
PBS, pH 7.4

Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Applications

Application Notes
WB: 1:500-1:1000, IHC: 1:200-1:800, ELISA: 1:8000

Target

Alternative Names
p52; p75; PAIP; DFS70; LEDGF; PSIP2

Gene ID
11168

UniProt ID
O75475

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-1024-XY415.pdf

MSDS

MSDS - VS-1024-XY415.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Recommended Dilution Buffer

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Isotype Control

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Product Name

Secondary Antibody

CAT
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DrMAB-487	Recombinant Human Anti-Human PSIP1 Antibody, clone D484	ELISA, IP-MS	IgG
MOB-1701CT	Recombinant Mouse anti-Human PSIP1 Monoclonal antibody (7F5)	ICC, IF, IHC-P, WB
VS3-XY1312	Mouse Anti-PSIP1 Recombinant Antibody (clone 6E4)	ELISA, WB, IHC, ICC	Mouse IgG1
VS3-XY3478	Rabbit Anti-PSIP1 Recombinant Antibody (clone R52-3N-5)	WB, IHC-P, ICC, IF	Rabbit IgG
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PABC-550	Recombinant Human Anti-PSIP1 Single Domain Antibody (PABC-550)	ELISA, WB, Inhib	Human VHH

CAT	Product Name	Application
VS-0325-XY1773	Anti-PSIP1 Immunohistochemistry Kit	IHC
VS-0525-XY5771	Anti-Mouse PSIP1 Immunohistochemistry Kit	IHC
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