Rabbit Anti-PTGS2 Recombinant Antibody (VS13-YC955)
CAT#: VS13-YC955
The Rabbit Anti-PTGS2 Recombinant Antibody is specific for human and rat PTGS2. PTGS2 encodes an inducible cyclooxygenase involved in prostaglandin biosynthesis, playing roles in inflammation and mitogenesis. The anti-PTGS2 antibody can be used for Western blot (1/500-1/5000), Immunocytochemistry (1/50-1/200), Immunofluorescence (1/50-1/200), Immunohistochemistry-paraffin (1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1335/990_H3_1_selected.jpg
Normal Tissue
Figure 2 Urinary bladder
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1335/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1335/4023_B_8_5.jpg
Normal Tissue
Figure 4 Seminal vesicle
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1335/4023_A_8_6.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enriched (BJ hTERT+)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000073756-PTGS2
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Specifications
- Immunogen
- Recombinant human COX2, aa 100-140.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat PTGS2
- Species Reactivity
- Human, Rat
- Applications
- WB, ICC, IF, IHC-P
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/5000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/100
Target
- Alternative Names
- Prostaglandin G/H Synthase 2
- Gene ID
- 5743
- UniProt ID
- P35354
- Long Name
- Prostaglandin-Endoperoxide Synthase 2
- Sequence Similarities
- Belongs to the prostaglandin G/H synthase family.
- Cellular Localization
- Endoplasmic reticulum; Cell membrane; Microsome; Nucleus
- Protein Refseq
- NP_000954.1
- Function
- Dual cyclooxygenase and peroxidase in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate, with a particular role in the inflammatory response (PubMed:7947975, PubMed:7592599, PubMed:9261177, PubMed:16373578, PubMed:22942274, PubMed:26859324, PubMed:27226593, PubMed:11939906, PubMed:19540099).
The cyclooxygenase activity oxygenates arachidonate (AA, C20:4(n-6)) to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide PGH2, the precursor of all 2-series prostaglandins and thromboxanes (PubMed:7947975, PubMed:7592599, PubMed:9261177, PubMed:16373578, PubMed:22942274, PubMed:26859324, PubMed:27226593).
This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons (PubMed:7947975, PubMed:7592599, PubMed:9261177, PubMed:16373578, PubMed:22942274, PubMed:26859324, PubMed:27226593).
Similarly catalyzes successive cyclooxygenation and peroxidation of dihomo-gamma-linoleate (DGLA, C20:3(n-6)) and eicosapentaenoate (EPA, C20:5(n-3)) to corresponding PGH1 and PGH3, the precursors of 1- and 3-series prostaglandins (PubMed:11939906, PubMed:19540099).
In an alternative pathway of prostanoid biosynthesis, converts 2-arachidonoyl lysophopholipids to prostanoid lysophopholipids, which are then hydrolyzed by intracellular phospholipases to release free prostanoids (PubMed:27642067).
Metabolizes 2-arachidonoyl glycerol yielding the glyceryl ester of PGH2, a process that can contribute to pain response (PubMed:22942274).
Generates lipid mediators from n-3 and n-6 polyunsaturated fatty acids (PUFAs) via a lipoxygenase-type mechanism. Oxygenates PUFAs to hydroperoxy compounds and then reduces them to corresponding alcohols (PubMed:11034610, PubMed:11192938, PubMed:9048568, PubMed:9261177).
Plays a role in the generation of resolution phase interaction products (resolvins) during both sterile and infectious inflammation (PubMed:12391014).
Metabolizes docosahexaenoate (DHA, C22:6(n-3)) to 17R-HDHA, a precursor of the D-series resolvins (RvDs) (PubMed:12391014).
As a component of the biosynthetic pathway of E-series resolvins (RvEs), converts eicosapentaenoate (EPA, C20:5(n-3)) primarily to 18S-HEPE that is further metabolized by ALOX5 and LTA4H to generate 18S-RvE1 and 18S-RvE2 (PubMed:21206090).
In vascular endothelial cells, converts docosapentaenoate (DPA, C22:5(n-3)) to 13R-HDPA, a precursor for 13-series resolvins (RvTs) shown to activate macrophage phagocytosis during bacterial infection (PubMed:26236990).
In activated leukocytes, contributes to oxygenation of hydroxyeicosatetraenoates (HETE) to diHETES (5, 15-diHETE and 5, 11-diHETE) (PubMed:22068350, PubMed:26282205).
During neuroinflammation, plays a role in neuronal secretion of specialized preresolving mediators (SPMs) 15R-lipoxin A4 that regulates phagocytic microglia (By similarity).
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Related Signaling Pathways
TNF Signaling Pathway
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "PTGS2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2188MZ | Recombinant Mouse Anti-Human PTGS2 Antibody (clone 5I23) | IHC-P | Mouse antibody |
| ZG-0201J | Mouse Anti-PTGS2 Recombinant Antibody (ZG-0201J) | IHC-P, IF | Mouse IgG |
| ZG-0202J | Mouse Anti-PTGS2 Recombinant Antibody (ZG-0202J) | IHC-P, IF | Mouse IgG |
| ZG-0203J | Mouse Anti-PTGS2 Recombinant Antibody (clone 3D4) | IHC-P, IF | Mouse IgG |
| ZG-0530U | Rabbit Anti-PTGS2 Recombinant Antibody (clone 4A9) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2922 | Hi-Affi™ Recombinant Rabbit Anti-PTGS2 Monoclonal Antibody (DS2922AB) | IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5837 | Anti-PTGS2 Immunohistochemistry Kit | IHC |
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