Mouse Anti-PTPN1 Recombinant Antibody (clone 4F11B9) (CAT#: VS7-0425-WR717)
This product is a mouse IgG1 antibody that binds to human PTPN1. The antibody 4F11B9 recognizes specific amino acid domain of human PTPN1 (AA: 40-246). It can be used for WB and FC assays.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12542/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12542/if_selected.jpg
Figure 2 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12542/30853_A_9_8_selected.jpg
(Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12542/30853_A_9_8_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_B_7_5.jpg
(Neuronal cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_B_7_5.jpg
Figure 4 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_A_7_5.jpg
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_A_7_5.jpg
Figure 5 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_A_9_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_A_9_8.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196396-PTPN1/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196396-PTPN1/subcellular
Specifications
- Immunogen
- Recombinant human PTPN1 (AA: 40-246) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PTPN1
- Species Reactivity
- Human
- Clone
- 4F11B9
- Molecule Class
- Non-Receptor Tyrosine Kinase
- Applications
- WB, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PTPN1; Protein-Tyrosine Phosphatase 1B; Protein Tyrosine Phosphatase, Placental; Tyrosine-Protein Phosphatase Non-Receptor Type 1; PTP-1B; EC 3.1.3.48; Protein Tyrosine Phosphatase, Non-Receptor Type 1; PTP1B
- Gene ID
- 5770
- UniProt ID
- P18031
- Long Name
- Protein tyrosine phosphatase non-receptor type 1
- Cellular Localization
- Endoplasmic reticulum; Membrane
- Post Translation Modifications
- Acetylation; Oxidation; Phosphoprotein; S-nitrosylation
- Protein Refseq
- NP_001265547.1
- Function
- Hydrolase; Protein phosphatase
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2288z | Mouse Anti-PTPN1 Recombinant Antibody (clone 24A4) | WB, FC, IF, IHC | Mouse IgG2a |
| MOB-0801CT | Recombinant Mouse anti-Human PTPN1 Monoclonal antibody (208BU700) | ELISA, WB | |
| HPAB-0374-CN | Chicken Anti-PTPN1 Recombinant Antibody (HPAB-0374-CN) | WB, ELISA, IP | Chicken IgY |
| VS3-FY1224 | Recombinant Rabbit Anti-PTPN1 Antibody (clone R05-3B9) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| VS3-WK2040 | Rabbit Anti-PTPN1 (pS378) Recombinant Antibody (clone R08-5F7) | WB, IHC-P | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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