Mouse Anti-PTPN1 Recombinant Antibody (clone 4F11B9)
CAT#: VS7-0425-WR717
This product is a mouse IgG1 antibody that binds to human PTPN1. The antibody 4F11B9 recognizes specific amino acid domain of human PTPN1 (AA: 40-246). It can be used for WB and FC assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12542/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12542/30853_A_9_8_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_B_7_5.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_A_7_5.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196396-PTPN1/subcellular
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Specifications
- Immunogen
- Recombinant human PTPN1 (AA: 40-246) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PTPN1
- Species Reactivity
- Human
- Clone
- 4F11B9
- Molecule Class
- Non-Receptor Tyrosine Kinase
- Applications
- WB, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PTPN1; Protein-Tyrosine Phosphatase 1B; Protein Tyrosine Phosphatase, Placental; Tyrosine-Protein Phosphatase Non-Receptor Type 1; PTP-1B; EC 3.1.3.48; Protein Tyrosine Phosphatase, Non-Receptor Type 1; PTP1B
- Gene ID
- 5770
- UniProt ID
- P18031
- Long Name
- Protein tyrosine phosphatase non-receptor type 1
- Cellular Localization
- Endoplasmic reticulum; Membrane
- Post Translation Modifications
- Acetylation; Oxidation; Phosphoprotein; S-nitrosylation
- Protein Refseq
- NP_001265547.1
- Function
- Hydrolase; Protein phosphatase
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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Secondary Antibody
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Reconstitution Buffer
- CAT
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Recommended Dilution Buffer
- CAT
- Product Name
See other products for "PTPN1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2288z | Mouse Anti-PTPN1 Recombinant Antibody (clone 24A4) | WB, FC, IF, IHC | Mouse IgG2a |
| HPAB-0374-CN | Chicken Anti-PTPN1 Recombinant Antibody (HPAB-0374-CN) | WB, ELISA, IP | Chicken IgY |
| HPAB-0375-CN | Chicken Anti-PTPN1 Recombinant Antibody (HPAB-0375-CN) | WB, ELISA, IP | Chicken IgY |
| MRO-2145-CN | Rabbit Anti-PTPN1 Polyclonal Antibody (MRO-2145-CN) | WB, IF, IHC | Rabbit IgG |
| MRO-2146-CN | Rabbit Anti-PTPN1 Polyclonal Antibody (MRO-2146-CN) | WB, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0455MZ | Chicken Anti-PTPN1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2928 | Hi-Affi™ Recombinant Rabbit Anti-PTPN1 Monoclonal Antibody (DS2928AB) | FC, ICC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0374-CN-S(P) | Chicken Anti-PTPN1 Recombinant Antibody; scFv Fragment (HPAB-0374-CN-S(P)) | WB, ELISA, IP | Chicken scFv |
| HPAB-0375-CN-S(P) | Chicken Anti-PTPN1 Recombinant Antibody; scFv Fragment (HPAB-0375-CN-S(P)) | WB, ELISA, IP | Chicken scFv |
| PSBC-544 | Chicken Anti-PTPN1 Recombinant Antibody scFv Fragment (PSBC-544) | ELISA | Chicken scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0374-CN-F(E) | Chicken Anti-PTPN1 Recombinant Antibody; Fab Fragment (HPAB-0374-CN-F(E)) | WB, ELISA, IP | Chicken Fab |
| HPAB-0375-CN-F(E) | Chicken Anti-PTPN1 Recombinant Antibody; Fab Fragment (HPAB-0375-CN-F(E)) | WB, ELISA, IP | Chicken Fab |
| PFBC-544 | Chicken Anti-PTPN1 Recombinant Antibody Fab Fragment (PFBC-544) | ELISA | Chicken Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC842 | AbPlus™ Anti-PTPN1 Magnetic Beads (VS-0724-YC842) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC420 | Recombinant Anti-PTPN1 Vesicular Antibody, EV Displayed (VS-0425-YC420) | ELISA, FC, Inhib, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5858 | Anti-PTPN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5859 | Anti-Rat PTPN1 Immunohistochemistry Kit | IHC |
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