Mouse Anti-PTPN1 Recombinant Antibody (clone 4F11B9) (CAT#: VS7-0425-WR717)
This product is a mouse IgG1 antibody that binds to human PTPN1. The antibody 4F11B9 recognizes specific amino acid domain of human PTPN1 (AA: 40-246). It can be used for WB and FC assays.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12542/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12542/if_selected.jpg
Figure 2 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12542/30853_A_9_8_selected.jpg
(Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12542/30853_A_9_8_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_B_7_5.jpg
(Neuronal cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_B_7_5.jpg
Figure 4 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_A_7_5.jpg
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_A_7_5.jpg
Figure 5 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_A_9_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/15217/36057_A_9_8.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196396-PTPN1/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196396-PTPN1/subcellular
Specifications
- Immunogen
- Recombinant human PTPN1 (AA: 40-246) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PTPN1
- Species Reactivity
- Human
- Clone
- 4F11B9
- Molecule Class
- Non-Receptor Tyrosine Kinase
- Applications
- WB, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PTPN1; Protein-Tyrosine Phosphatase 1B; Protein Tyrosine Phosphatase, Placental; Tyrosine-Protein Phosphatase Non-Receptor Type 1; PTP-1B; EC 3.1.3.48; Protein Tyrosine Phosphatase, Non-Receptor Type 1; PTP1B
- Gene ID
- 5770
- UniProt ID
- P18031
- Long Name
- Protein tyrosine phosphatase non-receptor type 1
- Cellular Localization
- Endoplasmic reticulum; Membrane
- Post Translation Modifications
- Acetylation; Oxidation; Phosphoprotein; S-nitrosylation
- Protein Refseq
- NP_001265547.1
- Function
- Hydrolase; Protein phosphatase
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2288z | Mouse Anti-PTPN1 Recombinant Antibody (clone 24A4) | WB, FC, IF, IHC | Mouse IgG2a |
| MOB-0801CT | Recombinant Mouse anti-Human PTPN1 Monoclonal antibody (208BU700) | ELISA, WB | |
| HPAB-0374-CN | Chicken Anti-PTPN1 Recombinant Antibody (HPAB-0374-CN) | WB, ELISA, IP | Chicken IgY |
| VS3-FY1224 | Recombinant Rabbit Anti-PTPN1 Antibody (clone R05-3B9) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| VS3-WK2040 | Rabbit Anti-PTPN1 (pS378) Recombinant Antibody (clone R08-5F7) | WB, IHC-P | Rabbit IgG |
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