Rabbit Anti-PYGO2 Recombinant Antibody (clone R08-6C7)
CAT#: VS3-XY3483
This rabbit recombinant antibody specifically recognizes PYGO2 (Pygopus family PHD finger 2), which involves in signal transduction through the Wnt pathway. The antibody specifically reacts with human and mouse PYGO2. It has been tested using Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western blot, Immunohistochemistry-Paraffin and Immunofluorescence is 1/500-1/1000, 1/50-1/100 and 1/50-1/200, respectively.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human oral mucosa
Immunohistochemical staining of human oral mucosa shows strong nuclear positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear
Glial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/52339_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/52339_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/52339_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/52339_A_4_6.jpg
Normal Tissue
Figure 7 Liver
Hepatocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/52339_A_7_4.jpg
Normal Tissue
Figure 8 Oral tissue
Squamous epithelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/52339_A_7_1.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/52339_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163348-PYGO2/subcellular
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse PYGO2
- Species Reactivity
- Human, Mouse
- Clone
- R08-6C7
- Applications
- WB, IHC-P, ICC, IF
- Related Disease
- Oligospermia; Azoospermia
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
- Alternative Names
- 1190004M21Rik
- Gene ID
- 90780
- UniProt ID
- Q9BRQ0
- Long Name
- Pygopus family PHD finger 2
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q9BRQ0)
Modification sites at neXtProt (NX_Q9BRQ0)
Glycosylation from GlyGen (Q9BRQ0) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_612157.1
- Function
- Involved in signal transduction through the Wnt pathway.
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Related Signaling Pathways
Canonical Wnt Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PYGO2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1732CT | Recombinant Mouse anti-Human PYGO2 Monoclonal antibody (QZHBE64B) | Dot, ELISA, WB | |
| MRO-2149-CN | Rabbit Anti-PYGO2 Polyclonal Antibody (MRO-2149-CN) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2949 | Hi-Affi™ Recombinant Rabbit Anti-PYGO2 Monoclonal Antibody (DS2949AB) | IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC459 | AbPlus™ Anti-PYGO2 Magnetic Beads (VS-0724-YC459) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5901 | Anti-PYGO2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5902 | Anti-Mouse PYGO2 Immunohistochemistry Kit | IHC |
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