Rabbit Anti-PYGO2 Recombinant Antibody (clone R08-6C7) (CAT#: VS3-XY3483)
This rabbit recombinant antibody specifically recognizes PYGO2 (Pygopus family PHD finger 2), which involves in signal transduction through the Wnt pathway. The antibody specifically reacts with human and mouse PYGO2. It has been tested using Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western blot, Immunohistochemistry-Paraffin and Immunofluorescence is 1/500-1/1000, 1/50-1/100 and 1/50-1/200, respectively.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/if_selected.jpg
Figure 2 IHC staining of human oral mucosa
(Immunohistochemical staining of human oral mucosa shows strong nuclear positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/ihc_selected.jpg
(Immunohistochemical staining of human oral mucosa shows strong nuclear positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/ihc_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear
Glial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/52339_B_8_5.jpg
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear
Glial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/52339_B_8_5.jpg
Figure 4 Colon
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/52339_A_9_3.jpg
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/52339_A_9_3.jpg
Figure 5 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/52339_A_8_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/52339_A_8_5.jpg
Figure 6 Testis
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/52339_A_4_6.jpg
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/52339_A_4_6.jpg
Figure 7 Liver
(Hepatocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/52339_A_7_4.jpg
(Hepatocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/52339_A_7_4.jpg
Figure 8 Oral tissue
(Squamous epithelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/52339_A_7_1.jpg
(Squamous epithelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/52339_A_7_1.jpg
Figure 9 Lymph node
(Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/52339_A_9_8.jpg
(Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23689/52339_A_9_8.jpg
Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163348-PYGO2/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163348-PYGO2/subcellular
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse PYGO2
- Species Reactivity
- Human, Mouse
- Clone
- R08-6C7
- Applications
- WB, IHC-P, ICC, IF
- Related Disease
- Oligospermia; Azoospermia
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
- Alternative Names
- 1190004M21Rik
- Gene ID
- 90780
- UniProt ID
- Q9BRQ0
- Long Name
- Pygopus family PHD finger 2
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q9BRQ0)
Modification sites at neXtProt (NX_Q9BRQ0)
Glycosylation from GlyGen (Q9BRQ0) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_612157.1
- Function
- Involved in signal transduction through the Wnt pathway.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Related Resources
Canonical Wnt Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1732CT | Recombinant Mouse anti-Human PYGO2 Monoclonal antibody (QZHBE64B) | Dot, ELISA, WB | |
| MRO-2149-CN | Rabbit Anti-PYGO2 Polyclonal Antibody (MRO-2149-CN) | WB, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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