Rabbit Anti-RAB25 Recombinant Antibody (VS13-YC967) (CAT#: VS13-YC967)
The Rabbit Anti-RAB25 Recombinant Antibody is specific for human RAB25. RAB25, involved in membrane trafficking and cell survival, acts as a tumor suppressor or oncogene contextually. The anti-RAB25 antibody can be used for Western blot (1/500-1/1000).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10872/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10872/if_selected.jpg
Figure 2 High expression in esophagus.
(Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_B_6_1.jpg
(Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_B_6_1.jpg
Figure 3 Colon
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_A_7_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_A_7_3.jpg
Figure 4 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_A_7_4.jpg
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_A_7_4.jpg
Figure 5 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_A_8_5.jpg
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_A_8_5.jpg
Figure 6 RNA cell line category: Cell line enhanced (BEWO, CAPAN-2, MCF7, OE19, RT4, SK-BR-3, T-47d)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132698-RAB25
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132698-RAB25
Specifications
- Immunogen
- Recombinant human Rab25.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human RAB25
- Species Reactivity
- Human
- Applications
- WB
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 150 mM NaCl and 50% Glycerol.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Target
- Alternative Names
- RAB25, Member RAS Oncogene Family; CATX-8; Ras-Related Protein Rab-25; RAB11C; CATX8;
- Gene ID
- 57111
- UniProt ID
- P57735
- Long Name
- RAB25, member RAS oncogene family
- Sequence Similarities
- Belongs to the small GTPase superfamily. Rab family.
- Cellular Localization
- Cell membrane; Cell projection; Cytoplasmic vesicle; Membrane
- Protein Refseq
- NP_065120.2
- Function
- Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia (PubMed:17925226).
Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions (By similarity).
May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis (By similarity).
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-044R | Mouse Anti-RAB25 Recombinant Antibody (ZG-044R) | WB, ELISA, IHC | Mouse IgG |
| VS3-XY1346 | Mouse Anti-RAB25 Recombinant Antibody (clone 3F12) | ELISA, WB, FC, IHC, ICC | Mouse IgG1 |
| VS3-XY1347 | Mouse Anti-RAB25 Recombinant Antibody (clone 3F12F3) | ELISA, WB, IHC | Mouse IgG2a |
| VS7-0425-WR737 | Mouse Anti-RAB25 Monoclonal Antibody (VS7-0425-WR737) | WB, IHC-P, IF, ICC, FC, ELISA | Mouse IgG |
| VS7-0425-WR738 | Mouse Anti-RAB25 Recombinant Antibody (VS7-0425-WR738) | WB, IHC-P, IF, ELISA | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
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