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    Mouse Anti-RAB25 Monoclonal Antibody (VS7-0425-WR737) (CAT#: VS7-0425-WR737)

    This product is a mouse IgG antibody that binds to human RAB25. The antibody can be used for WB, IHC-P, IF, ICC, FC, and ELISA assays. It cross reacts with mouse.

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    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

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    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line A-431
    Figure 1 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/10872/if_selected.jpg
    Normal Tissue
    Figure 2 High expression in esophagus.
    Figure 2 High expression in esophagus.
    (Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_B_6_1.jpg
    Figure 3 Colon
    Figure 3 Colon
    (Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_A_7_3.jpg
    Figure 4 Liver
    Figure 4 Liver
    (Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_A_7_4.jpg
    Figure 5 Kidney
    Figure 5 Kidney
    (Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_A_8_5.jpg
    RNA Expression
    Figure 6 RNA cell line category: Cell line enhanced (BEWO, CAPAN-2, MCF7, OE19, RT4, SK-BR-3, T-47d)
    Figure 6 RNA cell line category: Cell line enhanced (BEWO, CAPAN-2, MCF7, OE19, RT4, SK-BR-3, T-47d)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132698-RAB25

    Specifications

    • Immunogen
    • Recombinant human RAB25 fragment expressed in E. Coli.
    • Host Species
    • Mouse
    • Type
    • Mouse IgG
    • Specificity
    • Human RAB25
    • Species Reactivity
    • Human, Mouse
    • Molecule Class
    • Small GTPases
    • Applications
    • WB, IHC-P, IF, ICC, FC, ELISA
    • Conjugate
    • Unconjugated

    Product Property

    • Clonality
    • Monoclonal
    • Purity
    • >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
    • Format
    • Liquid
    • Concentration
    • 1 mg/ml
    • Preservative
    • 0.03% sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • Western Blot: 1:500 - 1:2000
      Immunohistochemistry: 1:200 - 1:1000
      Immunofluorescence: 1:200 - 1:1000
      Flow Cytometry: 1:200 - 1:400

    Target

    • Alternative Names
    • CATX-8; RAB11C
    • Long Name
    • RAB25, member RAS oncogene family
    • Cellular Localization
    • Cell membrane; Cell projection; Cytoplasmic vesicle; Membrane
    • Post Translation Modifications
    • Lipoprotein; Methylation; Prenylation
    • Function
    • Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia (PubMed:17925226).; Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions (By similarity).; May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis (By similarity).

    Recommended Products

    Isotype Control

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "RAB25"

    Select a product category from the dropdown menu below to view related products.
    Please select product type
    IgG Antibodies
    CAT Product Name Application Type
    ZG-044R Mouse Anti-RAB25 Recombinant Antibody (ZG-044R) WB, ELISA, IHC Mouse IgG
    VS3-XY1346 Mouse Anti-RAB25 Recombinant Antibody (clone 3F12) ELISA, WB, FC, IHC, ICC Mouse IgG1
    VS3-XY1347 Mouse Anti-RAB25 Recombinant Antibody (clone 3F12F3) ELISA, WB, IHC Mouse IgG2a
    VS4-CJ99 Recombinant Rabbit Anti-RAB25 Antibody (clone 27H3) ELISA, IF Rabbit IgG
    VS7-0425-WR738 Mouse Anti-RAB25 Recombinant Antibody (VS7-0425-WR738) WB, IHC-P, IF, ELISA Mouse IgG

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