Mouse Anti-RAB25 Monoclonal Antibody (VS7-0425-WR737)
CAT#: VS7-0425-WR737
This product is a mouse IgG antibody that binds to human RAB25. The antibody can be used for WB, IHC-P, IF, ICC, FC, and ELISA assays. It cross reacts with mouse.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10872/if_selected.jpg
Normal Tissue
Figure 2 High expression in esophagus.
Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_B_6_1.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_A_8_5.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (BEWO, CAPAN-2, MCF7, OE19, RT4, SK-BR-3, T-47d)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132698-RAB25
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Specifications
- Immunogen
- Recombinant human RAB25 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human RAB25
- Species Reactivity
- Human, Mouse
- Molecule Class
- Small GTPases
- Applications
- WB, IHC-P, IF, ICC, FC, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:200 - 1:1000
Immunofluorescence: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Target
- Alternative Names
- CATX-8; RAB11C
- Gene ID
- 57111
- UniProt ID
- P57735
- Long Name
- RAB25, member RAS oncogene family
- Cellular Localization
- Cell membrane; Cell projection; Cytoplasmic vesicle; Membrane
- Post Translation Modifications
- Lipoprotein; Methylation; Prenylation
- Protein Refseq
- NP_065120.2
- Function
- Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia (PubMed:17925226).; Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions (By similarity).; May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "RAB25"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-044R | Mouse Anti-RAB25 Recombinant Antibody (ZG-044R) | WB, ELISA, IHC | Mouse IgG |
| VS3-XY1346 | Mouse Anti-RAB25 Recombinant Antibody (clone 3F12) | ELISA, WB, FC, IHC, ICC | Mouse IgG1 |
| VS3-XY1347 | Mouse Anti-RAB25 Recombinant Antibody (clone 3F12F3) | ELISA, WB, IHC | Mouse IgG2a |
| VS4-CJ99 | Recombinant Rabbit Anti-RAB25 Antibody (clone 27H3) | ELISA, IF | Rabbit IgG |
| VS13-YC967 | Rabbit Anti-RAB25 Recombinant Antibody (VS13-YC967) | WB | Rabbit IgG |
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