Recombinant Rabbit Anti-RAB25 Antibody (clone 27H3) (CAT#: VS4-CJ99)
This rabbit anti-RAB25 antibody (clone 27H3) is a product that recognizes human RAB25. The immunogen of this antibody is a synthesized peptide derived from human RAB25. The mAb has been tested for use in ELISA, Immunofluorescence (1:50-1:200).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10872/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10872/if_selected.jpg
Figure 2 High expression in esophagus.
(Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_B_6_1.jpg
(Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_B_6_1.jpg
Figure 3 Colon
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_A_7_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_A_7_3.jpg
Figure 4 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_A_7_4.jpg
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_A_7_4.jpg
Figure 5 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_A_8_5.jpg
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/33645/157008_A_8_5.jpg
Figure 6 RNA cell line category: Cell line enhanced (BEWO, CAPAN-2, MCF7, OE19, RT4, SK-BR-3, T-47d)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132698-RAB25
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132698-RAB25
Specifications
- Immunogen
- A synthesized peptide derived from human RAB25
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human RAB25
- Species Reactivity
- Human
- Clone
- 27H3
- Applications
- ELISA, IF
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 150 mM NaCl, PBS, pH 7.4.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Immunofluorescence (1:50-1:200).
Target
- Alternative Names
- CATX-8; RAB11C
- Gene ID
- P57735
- UniProt ID
- P57735
- Long Name
- RAB25, member RAS oncogene family
- Sequence Similarities
- Belongs to the small GTPase superfamily. Rab family.
- Cellular Localization
- Cell membrane, Cell projection, Cytoplasmic vesicle, Membrane
- Protein Refseq
- NP_065120.2
- Function
- Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia (PubMed:17925226).
Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions (By similarity).
May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "RAB25"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-044R | Mouse Anti-RAB25 Recombinant Antibody (ZG-044R) | WB, ELISA, IHC | Mouse IgG |
| VS3-XY1346 | Mouse Anti-RAB25 Recombinant Antibody (clone 3F12) | ELISA, WB, FC, IHC, ICC | Mouse IgG1 |
| VS3-XY1347 | Mouse Anti-RAB25 Recombinant Antibody (clone 3F12F3) | ELISA, WB, IHC | Mouse IgG2a |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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