Mouse Anti-Rab5a Recombinant Antibody (clone 3H5C11)
CAT#: VS7-0425-WR744
This product is a mouse IgG1 antibody that binds to human Rab5a. The antibody 3H5C11 recognizes specific amino acid domain of human Rab5a (AA: 1-215). It can be used for WB, IHC, ICC, and FC assays.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human placenta
Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49354/122779_A_1_7_selected.jpg
Normal Tissue
Figure 2 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49354/122779_A_7_8.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49354/122779_B_8_5.jpg
Normal Tissue
Figure 4 Parathyroid gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49354/122779_A_3_8.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49354/122779_A_8_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49354/122779_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49354/122779_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49354/122779_A_4_6.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000144566-RAB5A
❮
❯
Specifications
- Immunogen
- Recombinant human Rab5a (AA: 1-215) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human Rab5a
- Species Reactivity
- Human
- Clone
- 3H5C11
- Molecule Class
- Small GTPases
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- RAB5A, Member RAS Oncogene Family; RAS-Associated Protein RAB5A; RAB5; Ras-Related Protein Rab-5A
- Gene ID
- 5868
- UniProt ID
- P20339
- Long Name
- RAB5A, Member RAS Oncogene Family
- Cellular Localization
- Cell membrane; Cell projection; Cytoplasm; Cytoplasmic vesicle; Endosome; Membrane
- Post Translation Modifications
- Glycoprotein; Lipoprotein; Phosphoprotein; Prenylation
- Protein Refseq
- NP_001278977.1
- Function
- Hydrolase
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS7-0425-WR744. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "RAB5A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1757CT | Recombinant Mouse anti-Human RAB5A Monoclonal antibody (EML666) | WB | |
| VS3-CJ771 | Rabbit Anti-RAB5A Recombinant Antibody (VS3-CJ771) | WB, IHC | Rabbit IgG |
| VS3-FY1232 | Recombinant Rabbit Anti-RAB5A Antibody (clone R01-6G5) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS3-FY1233 | Recombinant Rabbit Anti-RAB5A Antibody (clone R06-4J3) | WB, IHC-P | Rabbit IgG |
| VS13-YC969 | Rabbit Anti-RAB5A Recombinant Antibody (VS13-YC969) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2966 | Hi-Affi™ Recombinant Rabbit Anti-RAB5A Monoclonal Antibody (DS2966AB) | WB, IHC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5930 | Anti-RAB5A Immunohistochemistry Kit | IHC | |
| VS-0525-XY5931 | Anti-Mouse RAB5A Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: WB, ELISA, FuncS
Application: ELISA, FC, WB, FuncS
Application: ELISA, FC
Application: FC
Application: ELISA, Block, WB, FC, IP
Application: ELISA, FC, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
