Recombinant Rabbit Anti-RAB5A Antibody (clone R06-4J3)
CAT#: VS3-FY1233
This product is a recombinant rabbit antibody that recognizes RAB5A. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The clone R06-4J3 is specific for human, mouse, rat RAB5A. The antigen is synthetic peptide of human rab5.
Gene Expression
Tested Data
Normal Tissue
Figure 1 IHC staining of human placenta
Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49354/122779_A_1_7_selected.jpg
Normal Tissue
Figure 2 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49354/122779_A_7_8.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49354/122779_B_8_5.jpg
Normal Tissue
Figure 4 Parathyroid gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49354/122779_A_3_8.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49354/122779_A_8_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49354/122779_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49354/122779_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49354/122779_A_4_6.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000144566-RAB5A
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WB
Figure 1. Recombinant Rabbit Anti-RAB5A Antibody (clone R06-4J3) in WB.
Western Blot analysis of Rab5 in K562, C6, 3T3, Hela lysates using Rab5 Antibody.
IHC-P
Figure 2. Recombinant Rabbit Anti-RAB5A Antibody (clone R06-4J3) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Rab5 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide of human Rab5.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat RAB5A
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-4J3
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- Calculated MW: 24 kDa; Observed MW: 24 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Target
- Alternative Names
- RAB5A, Member RAS Oncogene Family; RAS-Associated Protein RAB5A; RAB5; Ras-Related Protein Rab-5A
- Gene ID
- 5868
- UniProt ID
- P20339
- Sequence Similarities
- Belongs to the small GTPase superfamily. Rab family.
- Cellular Localization
- Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Endosome, Membrane
- Post Translation Modifications
- Phosphorylation of Ser-84 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including RAB GDP dissociation inhibitors GDI1 and GDI2.
(Microbial infection) Glycosylated on arginine residues by S.typhimurium protein Ssek3.
- Protein Refseq
- NP_001278977.1; NP_004153.2
- Function
- Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032).
Contributes to the regulation of filopodia extension (PubMed:14978216).
Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413).
Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1757CT | Recombinant Mouse anti-Human RAB5A Monoclonal antibody (EML666) | WB | |
| MRO-1304-CN | Recombinant Rabbit Anti-RAB5A Monoclonal Antibody (CBACN-471) | WB, IHC | Rabbit IgG |
| VS3-CJ771 | Rabbit Anti-RAB5A Recombinant Antibody (VS3-CJ771) | WB, IHC | Rabbit IgG |
| VS3-FY1232 | Recombinant Rabbit Anti-RAB5A Antibody (clone R01-6G5) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS13-YC969 | Rabbit Anti-RAB5A Recombinant Antibody (VS13-YC969) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2966 | Hi-Affi™ Recombinant Rabbit Anti-RAB5A Monoclonal Antibody (DS2966AB) | WB, IHC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5930 | Anti-RAB5A Immunohistochemistry Kit | IHC | |
| VS-0525-XY5931 | Anti-Mouse RAB5A Immunohistochemistry Kit | IHC |
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