Rabbit Anti-RAPGEF3 Recombinant Antibody (VS13-YC979)
CAT#: VS13-YC979
The Rabbit Anti-RAPGEF3 Recombinant Antibody is specific for human and mouse RAPGEF3. RAPGEF3 encodes a guanine nucleotide exchange factor affecting pathways like platelet cytosolic Ca2+ response, linked to diseases like asthma. The anti-RAPGEF3 antibody can be used for Western blot (1/500-1/1000), Immunohistochemistry-paraffin (1/50-1/200), Immunoprecipitation (1/10-1/50), Immunocytochemistry (1/10-1/50), Immunofluorescence (1/10-1/50).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol & endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4386/672_D11_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43518/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in a subset of reaction center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4386/ihc_selected.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40365/103989_A_5_6.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (EFO-21, HUVEC TERT2, NB-4, RPTEC TERT1, TIME)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000079337-RAPGEF3
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Specifications
- Immunogen
- Recombinant human Epac1, aa 874-923.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse RAPGEF3
- Species Reactivity
- Human, Mouse
- Applications
- WB, IHC-P, IP, ICC, IF
- Conjugate
- NONE
- Related Disease
- Thrombocytopenia, Cyclic and Asthma
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Immunoprecipitation (IP): 1/10-1/50
Immunocytochemistry (ICC): 1/10-1/50
Immunofluorescence (IF): 1/10-1/50
Target
- Alternative Names
- Rap Guanine Nucleotide Exchange Factor 3; Exchange Protein Directly Activated By CAMP 1; Rap1 Guanine-Nucleotide-Exchange Factor Directly Activated By CAMP; CAMP-Regulated Guanine Nucleotide Exchange Factor I; Rap Guanine Nucleotide Exchange Factor (GEF) 3; Exchange Factor Directly Activated By CAMP 1; CAMP-GEFI; EPAC 1;
- Gene ID
- 10411
- UniProt ID
- O95398
- Long Name
- Rap Guanine Nucleotide Exchange Factor 3
- Cellular Localization
- Endomembrane system
- Function
- Signal transduction
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "RAPGEF3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1390MZ | Recombinant Mouse Anti-Human RAPGEF3 Antibody | ELISA, WB | Mouse antibody |
| MRO-0536-CN | Recombinant Rabbit Anti-RAPGEF3 Monoclonal Antibody (JM63-29) | WB, IHC, IP, IF | Rabbit IgG |
| VS3-XY1359 | Mouse Anti-RAPGEF3 Recombinant Antibody (clone 4G3) | WB, IP, IF, IHC | Mouse IgG1 |
| VS3-FY1249 | Recombinant Rabbit Anti-RAPGEF3 Antibody (clone R05-6B2) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3000 | Hi-Affi™ Recombinant Rabbit Anti-RAPGEF3 Monoclonal Antibody (DS3000AB) | WB, IP, IHC-P, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5986 | Anti-RAPGEF3 Immunohistochemistry Kit | IHC |
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