Anti-Rat HSPA5 Immunohistochemistry Kit
CAT#: VS-0525-XY3327
The HSPA5 IHC kit provides complete reagents for detecting this key ER chaperone involved in protein folding and the unfolded protein response. Suitable for paraffin and frozen tissues from various species, it supports research on cancer, metabolic disorders, and cellular stress responses with clear and consistent staining.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/if_selected.jpg
Normal Tissue
Figure 2 Colon
Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_9_3.jpg
Normal Tissue
Figure 3 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_9_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_7_8.jpg
Normal Tissue
Figure 7 Thyroid gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_B_3_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000044574-HSPA5
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Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human, Mouse, Rat
- Target
- HSPA5
- Primary Antibody
- Rabbit Anti-HSPA5 Antibody
- Secondary Antibody
- Goat anti-Rabbit Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Datasheet
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COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| TAB-009CT-F(E) | Mouse Anti-HSPA5 Recombinant Antibody; Fab Fragment (TAB-009CT-F(E)) | ELISA, Inhib, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| NS-035CN | Mouse Anti-HSPA5 Recombinant Antibody (NS-035CN) | FC | Mouse IgM |
| CAT | Product Name | Application | Type |
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| NS-035CN-F(E) | Mouse Anti-HSPA5 Recombinant Antibody; Fab Fragment (NS-035CN-F(E)) | FC | Mouse Fab |
| CAT | Product Name | Application | Type |
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| NS-035CN-S(P) | Mouse Anti-HSPA5 Recombinant Antibody; scFv Fragment (NS-035CN-S(P)) | FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0700-CN | Mouse Anti-HSPA5 Recombinant Antibody (clone C9-9) | WB, IF, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0368-YJ-S(P) | Mouse Anti-HSPA5 Recombinant Antibody scFv Fragment (HPAB-0368-YJ-S(P)) | WB, FC, IHC, ELISA, Inhib, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0369-YJ-S(P) | Human Anti-HSPA5 Recombinant Antibody scFv Fragment (HPAB-0369-YJ-S(P)) | WB, FC, IHC, ELISA, Inhib, FuncS | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0368-YJ-F(E) | Mouse Anti-HSPA5 Recombinant Antibody; Fab Fragment (HPAB-0368-YJ-F(E)) | WB, FC, IHC, ELISA, Inhib, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0369-YJ-F(E) | Human Anti-HSPA5 Recombinant Antibody; Fab Fragment (HPAB-0369-YJ-F(E)) | WB, FC, IHC, ELISA, Inhib, FuncS | Humanized Fab |
| CAT | Product Name | Application | Type |
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| VS-1024-XY234 | Mouse Anti-NHP HSPA5 Recombinant Antibody (clone 6H4-2G7) | WB, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| VS-0325-FY122 | Human Anti-HSPA5 (clone Sam-6) scFv-Fc Chimera | WB, FC, IF, Inhib, ELISA, IHC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
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| VS-0525-XY3325 | Anti-HSPA5 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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| VS-0525-XY3326 | Anti-Mouse HSPA5 Immunohistochemistry Kit | IHC |
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