Mouse Anti-HSPA5 Recombinant Antibody (clone 23C12)
CAT#: MOB-1425z
This product is a mouse antibody that recognizes human HSPA5. It can be used in the following applications: WB, ICC, IF.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/if_selected.jpg
Normal Tissue
Figure 2 Colon
Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_9_3.jpg
Normal Tissue
Figure 3 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_9_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_7_8.jpg
Normal Tissue
Figure 7 Thyroid gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_B_3_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000044574-HSPA5
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Specifications
- Immunogen
- Human Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa)
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human HSPA5
- Species Reactivity
- Human
- Clone
- 23C12
- Applications
- WB, ICC, IF
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western blot, Immunocytochemistry, Immunofluorescence.
Target
- Alternative Names
- HSPA5; GRP78; BIP; Endoplasmic Reticulum Lumenal Ca(2+)-Binding Protein Grp78; Heat Shock 70 KDa Protein 5; Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa); 78 KDa Glucose-Regulated Protein; GRP-78; MIF2; Heat Shock 70kD Protein 5 (Glucose-R
- UniProt ID
- P11021
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "HSPA5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-009CT | Mouse Anti-HSPA5 Recombinant Antibody (TAB-009CT) | ELISA, Inhib, FC | Mouse IgG1 |
| TAB-009CT-S(P) | Mouse Anti-HSPA5 Recombinant Antibody; scFv Fragment (TAB-009CT-S(P)) | ELISA, Inhib, FC | Mouse scFv |
| TAB-009CT-F(E) | Mouse Anti-HSPA5 Recombinant Antibody; Fab Fragment (TAB-009CT-F(E)) | ELISA, Inhib, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1695 | Rabbit Anti-HSPA5 Recombinant Antibody (clone DS1695AB) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-035CN | Mouse Anti-HSPA5 Recombinant Antibody (NS-035CN) | FC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-035CN-F(E) | Mouse Anti-HSPA5 Recombinant Antibody; Fab Fragment (NS-035CN-F(E)) | FC | Mouse Fab |
| NS-035CN-S(P) | Mouse Anti-HSPA5 Recombinant Antibody; scFv Fragment (NS-035CN-S(P)) | FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0699-CN | Mouse Anti-HSPA5 Recombinant Antibody (clone 2-7) | WB, IF, IHC | Mouse IgG1 |
| MRO-0788-CN | Mouse Anti-HSPA5 Recombinant Antibody (clone A5-C9-G6) | WB, IF, IHC, FC | Mouse IgG |
| ZG-0085C | Mouse Anti-HSPA5 Recombinant Antibody (clone 10C9) | WB, IHC-P, IF | Mouse IgG |
| ZG-0086C | Mouse Anti-HSPA5 Recombinant Antibody (clone 8G3) | WB, IHC-P, IF | Mouse IgG |
| VS3-FY2739 | Mouse Anti-HSPA5 Recombinant Antibody (VS3-FY2739) | WB, ICC, IP | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0368-YJ-S(P) | Mouse Anti-HSPA5 Recombinant Antibody (clone MAb159); scFv Fragment | WB, FC, IHC, ELISA, Inhib, FuncS | Mouse scFv |
| HPAB-0369-YJ-S(P) | Human Anti-HSPA5 Recombinant Antibody (clone huMAb159); scFv Fragment | WB, FC, IHC, ELISA, Inhib, FuncS | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0368-YJ-F(E) | Mouse Anti-HSPA5 Recombinant Antibody (clone MAb159); Fab Fragment | WB, FC, IHC, ELISA, Inhib, FuncS | Mouse Fab |
| HPAB-0369-YJ-F(E) | Human Anti-HSPA5 Recombinant Antibody (clone huMAb159); Fab Fragment | WB, FC, IHC, ELISA, Inhib, FuncS | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY234 | Mouse Anti-NHP HSPA5 Recombinant Antibody (clone 6H4-2G7) | WB, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY122 | Human Anti-HSPA5 (clone Sam-6) scFv-Fc Chimera | WB, FC, IF, Inhib, ELISA, IHC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3325 | Anti-HSPA5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3326 | Anti-Mouse HSPA5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3327 | Anti-Rat HSPA5 Immunohistochemistry Kit | IHC |
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