Human Anti-HSPA5 (clone Sam-6) scFv-Fc Chimera
CAT#: VS-0325-FY122
An anti-HSPA5 scFv-Fc antibody is a recombinant antibody that combines the antigen-binding capabilities of an scFv with the effector functions of an Fc region. The HSPA5 protein is a member of the heat shock protein family A (Hsp70).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/if_selected.jpg
Normal Tissue
Figure 2 Colon
Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_9_3.jpg
Normal Tissue
Figure 3 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_9_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_7_8.jpg
Normal Tissue
Figure 7 Thyroid gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_B_3_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000044574-HSPA5
❮
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Specifications
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- Human HSPA5
- Species Reactivity
- Human
- Clone
- Sam-6
- Applications
- Western Blot, Flow Cytometry, Immunofluorescence, Inhibition, ELISA, Immunohistochemistry
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Heat Shock Protein Family A (Hsp70) Member 5; Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa); Endoplasmic Reticulum Lumenal Ca(2+)-Binding Protein Grp78; Immunoglobulin Heavy Chain-Binding Protein; Glucose-Regulated Protein, 78kDa; GRP78; BIP; Heat Shock 70kD Protein 5 (Glucose-Regulated Protein, 78kD)
- Gene ID
- 3309
- UniProt ID
- P11021
- Long Name
- Heat Shock Protein Family A (Hsp70) Member 5
- Sequence Similarities
- Belongs to the heat shock protein 70 family.
- Cellular Localization
- Endoplasmic reticulum lumen; Melanosome; Cytoplasm; Cell surface
- Post Translation Modifications
- AMPylated by FICD.
In unstressed cells, AMPylation at Thr-518 by FICD inactivates the chaperome activity: AMPylated form is locked in a relatively inert state and only weakly stimulated by J domain-containing proteins (By similarity).
In response to endoplasmic reticulum stress, de-AMPylation by the same protein, FICD, restores the chaperone activity (By similarity).
Glycosylation at Thr69, Thr203, and Thr643
Ubiquitination at Lys96, Lys113, Lys118, Lys213, Lys268, Lys326, Lys370, Lys376, Lys523, Lys547, Lys573, and Lys601
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 6 sites, 2 O-linked glycans (5 sites)
- Protein Refseq
- NP_005338.1
- Function
- Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) (PubMed:1550958, 19538957).
In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1 (By similarity).
Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1 (By similarity).
Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER).
May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| NS-035CN-F(E) | Mouse Anti-HSPA5 Recombinant Antibody; Fab Fragment (NS-035CN-F(E)) | FC | Mouse Fab |
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| CAT | Product Name | Application | Type |
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| HPAB-0368-YJ-S(P) | Mouse Anti-HSPA5 Recombinant Antibody (clone MAb159); scFv Fragment | WB, FC, IHC, ELISA, Inhib, FuncS | Mouse scFv |
| HPAB-0369-YJ-S(P) | Human Anti-HSPA5 Recombinant Antibody (clone huMAb159); scFv Fragment | WB, FC, IHC, ELISA, Inhib, FuncS | Humanized scFv |
| CAT | Product Name | Application | Type |
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| HPAB-0368-YJ-F(E) | Mouse Anti-HSPA5 Recombinant Antibody (clone MAb159); Fab Fragment | WB, FC, IHC, ELISA, Inhib, FuncS | Mouse Fab |
| HPAB-0369-YJ-F(E) | Human Anti-HSPA5 Recombinant Antibody (clone huMAb159); Fab Fragment | WB, FC, IHC, ELISA, Inhib, FuncS | Humanized Fab |
| CAT | Product Name | Application | Type |
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| ZG-0085C | Mouse Anti-HSPA5 Recombinant Antibody (clone 10C9) | WB, IHC-P, IF | Mouse IgG |
| ZG-0086C | Mouse Anti-HSPA5 Recombinant Antibody (clone 8G3) | WB, IHC-P, IF | Mouse IgG |
| ZG-0448J | Mouse Anti-HSPA5 Recombinant Antibody (ZG-0448J) | WB, IF | Mouse IgG |
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| VS3-CJ582 | Mouse Anti-HSPA5 Recombinant Antibody (VS3-CJ582) | WB, IHC | Mouse IgG |
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| VS-1024-XY234 | Mouse Anti-NHP HSPA5 Recombinant Antibody (clone 6H4-2G7) | WB, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| VS-0525-XY3326 | Anti-Mouse HSPA5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3327 | Anti-Rat HSPA5 Immunohistochemistry Kit | IHC |
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