Mouse Anti-RICTOR Monoclonal Antibody (VS7-0425-WR781)
CAT#: VS7-0425-WR781
This product is a mouse IgG antibody that binds to human RICTOR. The antibody can be used for WB, IHC-P, IF, ICC, FC, and ELISA assays. It cross reacts with mouse and monkey.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37802/if_selected.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37802/74753_A_8_3.jpg
Normal Tissue
Figure 3 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37802/74753_A_7_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37802/74753_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37802/74753_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37802/74753_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000164327-RICTOR
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Specifications
- Immunogen
- Recombinant human RICTOR fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human RICTOR
- Species Reactivity
- Human, Mouse, Monkey
- Molecule Class
- Serine/Threonine Kinase
- Applications
- WB, IHC-P, IF, ICC, FC, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:200 - 1:1000
Immunofluorescence: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Target
- Alternative Names
- RPTOR Independent Companion Of MTOR Complex 2; Rapamycin-Insensitive Companion Of MTOR; AVO3 Homolog; Pianissimo; HAVO3; RPTOR Independent Companion Of MTOR, Complex 2
- Gene ID
- 253260
- UniProt ID
- Q6R327
- Long Name
- RPTOR Independent Companion Of MTOR Complex 2
- Cellular Localization
- Cell membrane; Endoplasmic reticulum; Lysosome; Membrane
- Post Translation Modifications
- Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001272368.1
- Function
- Developmental protein
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Related Signaling Pathways
mTOR Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "RICTOR"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1650MZ | Recombinant Mouse Anti-Human RICTOR Antibody | ICC, IF, IHC-P, WB | Mouse antibody |
| ZG-069R | Mouse Anti-RICTOR Recombinant Antibody (ZG-069R) | WB, ELISA | Mouse IgG |
| VS3-QX972 | Mouse Anti-RICTOR Recombinant Antibody (clone 4H5) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-WK65 | Mouse Anti-RICTOR Recombinant Antibody (clone E9-B3) | WB, ELISA | Mouse IgG1 |
| VS3-XY1372 | Mouse Anti-RICTOR Recombinant Antibody (clone 7B3) | ELISA, WB, FC, IHC, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY448 | Rabbit Anti-NHP RICTOR Recombinant Antibody (VS-1024-XY448) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1005 | CytoStream™ Rabbit Anti-RICTOR Recombinant Antibody (VS13-YC1005) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1921 | Anti-RICTOR Immunohistochemistry Kit | IHC | |
| VS-0525-XY6125 | Anti-Rat RICTOR Immunohistochemistry Kit | IHC | |
| VS-0525-XY6124 | Anti-Human RICTOR Immunohistochemistry Kit | IHC |
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