Mouse Anti-Ring1 Recombinant Antibody (clone 8C12F4) (CAT#: VS7-0425-WR782)

Name: Mouse Anti-Ring1 Recombinant Antibody (clone 8C12F4)
SKU: VS7-0425-WR782
Rating: 5 (1 reviews)

This product is a mouse IgG1 antibody that binds to human Ring1. The antibody 8C12F4 recognizes specific amino acid domain of human RIPK1 (AA: 131-277). It can be used for WB, IHC, and FC assays.

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Gene Expression
Datasheet
MSDS
COA

Subcellular Location

Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8701/if_selected.jpg

Normal Tissue

Figure 2 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8701/23720_B_9_5.jpg

Figure 3 Nasopharynx
(Respiratory epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8701/23720_B_7_2.jpg

Figure 4 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8701/23720_A_9_3.jpg

Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8701/23720_A_7_5.jpg

Figure 6 Testis
(Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Peritubular cells Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8701/23720_A_4_6.jpg

Figure 7 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8701/23720_A_8_8.jpg

RNA Expression

Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204227-RING1

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Recombinant human Ring1 (AA: 79-263) fragment expressed in E. Coli.

Host Species
Mouse

Type
Mouse IgG1

Specificity
Human Ring1

Species Reactivity
Human

Clone
8C12F4

Molecule Class
E3 Ubiquitin-Protein Ligase

Applications
WB, IHC, FC

Conjugate
Unconjugated

Product Property

Clonality
Monoclonal

Purity
>95% as determined by SDS-PAGE and SEC-HPLC analysis

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
0.05% sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000

Target

Alternative Names
RING1; E3 Ubiquitin-Protein Ligase RING1; RING Finger Protein 1; Polycomb Complex Protein RING1; Ring Finger Protein 1; RING1A; Really Interesting New Gene 1 Protein; EC 6.3.2.-; EC 6.3.2; RNF1

Gene ID
6015

UniProt ID
Q06587

Long Name
Ring Finger Protein 1

Cellular Localization
Nucleus

Post Translation Modifications
Phosphoprotein

Protein Refseq
NP_002922.2

Function
Chromatin regulator; Repressor; Transferase

Isotype Control

Secondary Antibody

Reconstitution Buffer

Recommended Dilution Buffer

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "RING1"

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IgG Antibody Products Rabbit Monoclonal Antibody Products

CAT	Product Name	Application	Type
MOB-2624z	Mouse Anti-RING1 Recombinant Antibody (clone 27F9)	WB, ELISA, FC, ICC, IF, IHC	Mouse IgG1
MOB-1653MZ	Recombinant Mouse Anti-Human RING1 Antibody	WB	Mouse antibody
VS3-QX973	Mouse Anti-RING1 Recombinant Antibody (clone 7D12-G1-B7)	WB, IHC, ICC, FC, ELISA	Mouse IgG1
VS3-WK82	Mouse Anti-RING1 Recombinant Antibody (clone F4-C12)	WB, ELISA	Mouse IgG1
VS3-XY1374	Mouse Anti-RING1 Recombinant Antibody (clone 8E8A2)	ELISA, WB, ICC	Mouse IgG1

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