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  • Rabbit Anti-RNF20 Recombinant Antibody (clone R09-2F6)

Rabbit Anti-RNF20 Recombinant Antibody (clone R09-2F6) (CAT#: VS3-XY3487)

It is a recombinant rabbit antibody specific for RNF20 (Ring finger protein 20), which mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation. This antibody specifically targets human and mouse RNF20. This product has been tested using Western Blot, Immunoprecipitation, Flow cytometry, Immunocytochemistry or Immunofluorescence.


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  • Datasheet
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51773/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human gall bladder
Figure 3 IHC staining of human gall bladder
(Immunohistochemical staining of human gall bladder shows distinct nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/ihc_selected.jpg
Figure 4 Cerebral cortex
Figure 4 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/27446_B_8_5.jpg
Figure 5 Colon
Figure 5 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/27446_A_7_3.jpg
Figure 6 Kidney
Figure 6 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/27446_A_7_5.jpg
Figure 7 Testis
Figure 7 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/27446_A_6_6.jpg
Figure 8 Lymph node
Figure 8 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/27446_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000155827-RNF20

Specifications

  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human and Mouse RNF20
  • Species Reactivity
  • Human, Mouse
  • Clone
  • R09-2F6
  • Applications
  • WB, ICC, IF, IP, FC
  • Related Disease
  • Testicular Germ Cell Cancer

Product Property

  • Clonality
  • Monoclonal
  • Aggregation
  • < 5%
  • Format
  • Liquid
  • Buffer
  • PBS (pH 7.4), 150 mM NaCl and 50% glycerol
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • WB: 1/500-1/1000
    IF: 1/50-1/200
    IP: 1/20
    FC: 1/50-1/100

Target

  • Alternative Names
  • BRE1; BRE1A; hBRE1
  • Long Name
  • Ring finger protein 20
  • Cellular Localization
  • Nucleus
  • Post Translation Modifications
  • Ubiquitination at Lys194, Lys242, and Lys821 (NX_Q5VTR2 [NX_Q5VTR2-1])
    Modification sites at PhosphoSitePlus (Q5VTR2)
    Glycosylation from GlyGen (Q5VTR2) 2 sites, 1 O-linked glycan (2 sites)
  • Function
  • Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1).
    H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively).
    It thereby plays a central role inb histone code and gene regulation.
    The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory.

Recommended Products

Isotype Control

Recommended Dilution Buffer

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "RNF20"

Recombinant Antibody

CAT Product Name Application Type
MOB-1045CT Recombinant Mouse anti-Human RNF20 Monoclonal antibody (SOG30-96) I-ELISA, ICC/IF, WB
MRO-1341-CN Recombinant Rabbit Anti-RNF20 Monoclonal Antibody (CBACN-486) WB, IP, FC Rabbit IgG
ZG-0098U Rabbit Anti-RNF20 Recombinant Antibody (clone 2H12) ELISA, WB, IHC, IF Rabbit IgG
VS3-WK1029 Mouse Anti-RNF20 Recombinant Antibody (VS3-WK1029) WB Mouse IgG
VS3-WK1030 Rabbit Anti-RNF20 Recombinant Antibody (VS3-WK1030) WB, IP, FC Rabbit IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-3085 Hi-Affi™ Recombinant Rabbit Anti-RNF20 Monoclonal Antibody (DS3085AB) ICC/IF, WB, FC, IP IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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