Rabbit Anti-RNF20 Recombinant Antibody (clone R09-2F6)
CAT#: VS3-XY3487
It is a recombinant rabbit antibody specific for RNF20 (Ring finger protein 20), which mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation. This antibody specifically targets human and mouse RNF20. This product has been tested using Western Blot, Immunoprecipitation, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51773/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human gall bladder
Immunohistochemical staining of human gall bladder shows distinct nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/27446_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/27446_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/27446_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/27446_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12478/27446_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000155827-RNF20
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse RNF20
- Species Reactivity
- Human, Mouse
- Clone
- R09-2F6
- Applications
- WB, ICC, IF, IP, FC
- Related Disease
- Testicular Germ Cell Cancer
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- BRE1; BRE1A; hBRE1
- Gene ID
- 56254
- UniProt ID
- Q5VTR2
- Long Name
- Ring finger protein 20
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitination at Lys194, Lys242, and Lys821 (NX_Q5VTR2 [NX_Q5VTR2-1])
Modification sites at PhosphoSitePlus (Q5VTR2)
Glycosylation from GlyGen (Q5VTR2) 2 sites, 1 O-linked glycan (2 sites)
- Protein Refseq
- XP_047279550.1; XP_011517164.1
- Function
- Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1).
H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively).
It thereby plays a central role inb histone code and gene regulation.
The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "RNF20"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1045CT | Recombinant Mouse anti-Human RNF20 Monoclonal antibody (SOG30-96) | I-ELISA, ICC, IF, WB | |
| MRO-1341-CN | Recombinant Rabbit Anti-RNF20 Monoclonal Antibody (CBACN-486) | WB, IP, FC | Rabbit IgG |
| ZG-0098U | Rabbit Anti-RNF20 Recombinant Antibody (clone 2H12) | ELISA, WB, IHC, IF | Rabbit IgG |
| VS3-WK1029 | Mouse Anti-RNF20 Recombinant Antibody (VS3-WK1029) | WB | Mouse IgG |
| VS3-WK1030 | Rabbit Anti-RNF20 Recombinant Antibody (VS3-WK1030) | WB, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3085 | Hi-Affi™ Recombinant Rabbit Anti-RNF20 Monoclonal Antibody (DS3085AB) | ICC, IF, WB, FC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1930 | Anti-RNF20 Immunohistochemistry Kit | IHC |
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