Anti-ROBO1 Antibody Prodrug, Protease Activated (VS-1025-YC133)
CAT#: VS-1025-YC133
The Anti-ROBO1 Antibody Prodrug, Protease Activated (VS-1025-YC133) is an antibody prodrug comprised of a recombinant mouse antibody targeting human ROBO1, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to ROBO1 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to ROBO1 effectively. ROBO1 (Roundabout Homolog 1) is a guidance receptor that is often considered a tumor suppressor. Loss of expression or inactivation of ROBO1 in cancers is associated with the promotion of cell migration, invasion, and metastasis, allowing the tumor cell to disregard normal positional cues.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52968/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human breast
Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/33705_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/33705_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/33705_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/33705_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/33705_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/33705_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169855-ROBO1
❮
❯
Specifications
- Host Animal
- Mouse
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human ROBO1
- Target
- ROBO1
- Species Reactivity
- Human
- Isotype
- IgG
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3.
- Alternative Names
- SAX3; DUTT1
- Gene ID
- 6091
- UniProt ID
- Q9Y6N7
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Datasheet
MSDS
COA
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See other products for "ROBO1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-346 | Mouse Anti-ROBO1 Recombinant Antibody (PABL-346) | WB, ELISA, FuncS | Mouse IgG2a kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-346 | Mouse Anti-ROBO1 Recombinant Antibody; scFv Fragment (PSBL-346) | WB, ELISA, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-346 | Mouse Anti-ROBO1 Recombinant Antibody; Fab Fragment (PFBL-346) | WB, ELISA, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0281CL | Anti-Human ROBO1 Recombinant Antibody (A7241A) | IB, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0282CL | Anti-Human ROBO1 Recombinant Antibody (B2318C (binds to extracellular region of ROBO1)) | Cyt, FC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0281CL-S(P) | Anti-Human ROBO1 Recombinant Antibody scFv Fragment (A7241A) | WB, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0282CL-S(P) | Anti-Human ROBO1 Recombinant Antibody scFv Fragment (B2318C (binds to extracellular region of ROBO1)) | WB, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0281CL-F(E) | Anti-Human ROBO1 Recombinant Antibody Fab Fragment (A7241A) | WB, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1275MZ | Recombinant Mouse Anti-Human ROBO1 Antibody (clone 2G6) | WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2166-CN | Rabbit Anti-ROBO1 Polyclonal Antibody (MRO-2166-CN) | IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABJ-0064 | Mouse Anti-ROBO1 Recombinant Antibody (PABJ-0064) | ELISA, FuncS | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABJ-0064-S(P) | Mouse Anti-ROBO1 Recombinant Antibody; scFv Fragment (PABJ-0064-S(P)) | ELISA, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABJ-0064-F(E) | Mouse Anti-ROBO1 Recombinant Antibody; Fab Fragment (PABJ-0064-F(E)) | ELISA, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY52 | Human Anti-ROBO1 scFv-Fc Chimera (VS-0125-FY52) | IA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC350 | Recombinant Anti-ROBO1 Vesicular Antibody, EV Displayed (VS-0425-YC350) | Cyt, ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6171 | Anti-ROBO1 Immunohistochemistry Kit | IHC |
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