Rabbit Anti-ROCK1 Recombinant Antibody (VS13-YC1013) (CAT#: VS13-YC1013)
The Rabbit Anti-ROCK1 Recombinant Antibody is specific for human and mouse ROCK1. ROCK1 encodes a serine/threonine kinase activated by Rho, influencing processes like fibroblast adhesion and platelet aggregation, with a related pseudogene on chromosome 18. The anti-ROCK1 antibody can be used for Western blot (1/500-1/5000), Immunocytochemistry (1/50-1/200), Immunofluorescence (1/50-1/200), Immunohistochemistry-paraffin (1/50-1/200).
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Normal Tissue
RNA Expression
Figure 1 Cerebral cortex
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7567/21573_B_7_5.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7567/21573_B_7_5.jpg
Figure 2 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7567/21573_A_9_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7567/21573_A_9_3.jpg
Figure 3 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7567/21573_A_7_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7567/21573_A_7_5.jpg
Figure 4 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7567/21573_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7567/21573_A_6_6.jpg
Figure 5 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7567/21573_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7567/21573_A_8_8.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000067900-ROCK1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000067900-ROCK1
Specifications
- Immunogen
- Recombinant human ROCK1, aa 1104-1149.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse ROCK1
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, IHC-P, IP
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/5000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Target
- Alternative Names
- Rho Associated Coiled-Coil Containing Protein Kinase 1; Renal Carcinoma Antigen NY-REN-35; P160 ROCK-1; EC 2.7.11.1; P160ROCK; ROCK-I;
- Gene ID
- 6093
- UniProt ID
- Q13464
- Long Name
- Rho Associated Coiled-Coil Containing Protein Kinase 1
- Cellular Localization
- Cell membrane; Cell projection; Cytoplasm; Cytoskeleton; Golgi apparatus
- Function
- Signal transduction
Recommended Products
Secondary Antibody
Isotype Control
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2650z | Mouse Anti-ROCK1 Recombinant Antibody (clone 6F2) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| MRO-1342-CN | Recombinant Rabbit Anti-ROCK1 Monoclonal Antibody (CBACN-487) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-2167-CN | Rabbit Anti-ROCK1 Polyclonal Antibody (MRO-2167-CN) | WB, IHC | Rabbit IgG |
| VS3-FY1275 | Recombinant Rabbit Anti-ROCK1 Antibody (clone R06-9A5) | WB, IP | Rabbit IgG |
| VS3-FY1276 | Recombinant Rabbit Anti-ROCK1 Antibody (clone R09-3D3) | WB, IP | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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