Rabbit Anti-ROCK1 Recombinant Antibody (VS13-YC1013) (CAT#: VS13-YC1013)
The Rabbit Anti-ROCK1 Recombinant Antibody is specific for human and mouse ROCK1. ROCK1 encodes a serine/threonine kinase activated by Rho, influencing processes like fibroblast adhesion and platelet aggregation, with a related pseudogene on chromosome 18. The anti-ROCK1 antibody can be used for Western blot (1/500-1/5000), Immunocytochemistry (1/50-1/200), Immunofluorescence (1/50-1/200), Immunohistochemistry-paraffin (1/50-1/200).
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Normal Tissue
RNA Expression
Figure 1 Cerebral cortex
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7567/21573_B_7_5.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7567/21573_B_7_5.jpg
Figure 2 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7567/21573_A_9_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7567/21573_A_9_3.jpg
Figure 3 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7567/21573_A_7_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7567/21573_A_7_5.jpg
Figure 4 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7567/21573_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7567/21573_A_6_6.jpg
Figure 5 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7567/21573_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7567/21573_A_8_8.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000067900-ROCK1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000067900-ROCK1
Specifications
- Immunogen
- Recombinant human ROCK1, aa 1104-1149.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse ROCK1
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, IHC-P, IP
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/5000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Target
- Alternative Names
- Rho Associated Coiled-Coil Containing Protein Kinase 1; Renal Carcinoma Antigen NY-REN-35; P160 ROCK-1; EC 2.7.11.1; P160ROCK; ROCK-I;
- Gene ID
- 6093
- UniProt ID
- Q13464
- Long Name
- Rho Associated Coiled-Coil Containing Protein Kinase 1
- Cellular Localization
- Cell membrane; Cell projection; Cytoplasm; Cytoskeleton; Golgi apparatus
- Function
- Signal transduction
Recommended Products
Secondary Antibody
Isotype Control
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2650z | Mouse Anti-ROCK1 Recombinant Antibody (clone 6F2) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| MRO-1342-CN | Recombinant Rabbit Anti-ROCK1 Monoclonal Antibody (CBACN-487) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-2167-CN | Rabbit Anti-ROCK1 Polyclonal Antibody (MRO-2167-CN) | WB, IHC | Rabbit IgG |
| VS3-FY1275 | Recombinant Rabbit Anti-ROCK1 Antibody (clone R06-9A5) | WB, IP | Rabbit IgG |
| VS3-FY1276 | Recombinant Rabbit Anti-ROCK1 Antibody (clone R09-3D3) | WB, IP | Rabbit IgG |
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