Rabbit Anti-ROCK2 Recombinant Antibody (clone 10E1) (CAT#: ZG-0549U)
Rabbit Anti-ROCK2 Antibody (clone 10E1) displayed high specificity against the ROCK2 protein of Human, Mouse and Rat.
Specific Inquiry
WB
Figure 1 Rabbit Anti-ROCK2 Antibody (ZG-0549U) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, L02 whole cell lysate, HepG2 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate
All lanes: ROCK2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 161 kDa
Observed band size: 161 kDa
IF
Figure 2 Rabbit Anti-ROCK2 Antibody (ZG-0549U) in IF
Immunofluorescence staining of Hela Cells with ZG-0549U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human ROCK2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ROCK2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 10E1
- Applications
- ELISA, WB, IF
- Conjugate
- Unconjugated
- Related Disease
- Dextrocardia and Ureteral Obstruction.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IF:1:20-1:200
Target
- Alternative Names
- Rho-associated protein kinase 2; EC 2.7.11.1; Rho kinase 2; Rho-associated; coiled-coil-containing protein kinase 2; Rho-associated; coiled-coil-containing protein kinase II; ROCK-II; p164 ROCK-2; ROCK2; KIAA0619
- Gene ID
- 9475
- UniProt ID
- O75116
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "ROCK2"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1277MZ | Recombinant Mouse Anti-Human ROCK2 Antibody | ICC/IF, IHC-P, IP, WB | Mouse antibody |
| MRO-2168-CN | Rabbit Anti-ROCK2 Polyclonal Antibody (MRO-2168-CN) | WB, IF, IHC | Rabbit IgG |
| VS3-FY1277 | Recombinant Rabbit Anti-ROCK2 Antibody (clone R01-4F1) | WB, ICC, IF | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3094 | Hi-Affi™ Recombinant Rabbit Anti-ROCK2 Monoclonal Antibody (DS3094AB) | WB, IHC, ICC | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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