Human Anti-Rotavirus VP8* Recombinant Antibody (clone mAb9) (CAT#: PABJ-0170)

This product is a Human antibody that recognizes Rotavirus VP8*. scFv9 binds to a site distant from the HBGA binding site. mAb9 neutralizes the virus is not by blocking the glycan binding.

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SDS-PAGE

Figure 1 Human Anti-Rotavirus VP8* Recombinant Antibody (clone mAb9) (PABJ-0170) in SDS-PAGE

Figure 1 Human Anti-Rotavirus VP8* Recombinant Antibody (clone mAb9) (PABJ-0170) in SDS-PAGE

SDS-PAGE analysis of PABJ-0170 in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as about 135-245 kDa.

WB

Figure 2 Human Anti-Rotavirus VP8* Recombinant Antibody (clone mAb9) (PABJ-0170) in WB

Figure 2 Human Anti-Rotavirus VP8* Recombinant Antibody (clone mAb9) (PABJ-0170) in WB

Western blot analysis of PABJ-0170 was performed by loading Rotavirus A VP4 Protein, His Tag.

PABJ-0170 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat anti-Human IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)

ELISA

Figure 3 Human Anti-Rotavirus VP8* Recombinant Antibody (clone mAb9) (PABJ-0170) in ELISA

Figure 3 Human Anti-Rotavirus VP8* Recombinant Antibody (clone mAb9) (PABJ-0170) in ELISA

ELISA analysis of PABJ-0170 was performed by coating with Rotavirus A VP4 Protein, His Tag.

The secondary antibody: HRP-Goat anti-Human IgG

DB

Figure 4 Human Anti-Rotavirus VP8* Recombinant Antibody (clone mAb9) (PABJ-0170) in DB

Figure 4 Human Anti-Rotavirus VP8* Recombinant Antibody (clone mAb9) (PABJ-0170) in DB

Dot Blot analysis of PABJ-0170 was performed by coating with Rotavirus A VP4 Protein, His Tag.

The secondary antibody: HRP-Goat anti-Human IgG


Specifications

  • Host Species
  • Human
  • Type
  • Human IgG
  • Specificity
  • Rotavirus VP8*
  • Species Reactivity
  • Rotavirus A
  • Clone
  • mAb9
  • Applications
  • ELISA, Neut

Product Property

  • Purification
  • Protein A/G Purified
  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Buffer
  • PBS
  • Storage
  • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Neutralization.

Target

  • Alternative Names
  • Rotavirus; VP8*; RV; Rotavirus A

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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CAT Product Name Application Type
PABJ-0170-S(P) Human Anti-Rotavirus VP8* Recombinant Antibody (clone mAb9); scFv Fragment ELISA, Neut Human scFv

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To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# PABJ-0170, RRID: AB_3111613)

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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