This product is a Human antibody that recognizes Rotavirus VP8*. scFv9 binds to a site distant from the HBGA binding site. mAb9 neutralizes the virus is not by blocking the glycan binding.
Figure 1 Human Anti-Rotavirus VP8* Recombinant Antibody (clone mAb9) (PABJ-0170) in SDS-PAGE
SDS-PAGE analysis of PABJ-0170 in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as about 135-245 kDa.
Figure 2 Human Anti-Rotavirus VP8* Recombinant Antibody (clone mAb9) (PABJ-0170) in WB
Western blot analysis of PABJ-0170 was performed by loading Rotavirus A VP4 Protein, His Tag.
PABJ-0170 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat anti-Human IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
Figure 3 Human Anti-Rotavirus VP8* Recombinant Antibody (clone mAb9) (PABJ-0170) in ELISA
ELISA analysis of PABJ-0170 was performed by coating with Rotavirus A VP4 Protein, His Tag.
The secondary antibody: HRP-Goat anti-Human IgG
Figure 4 Human Anti-Rotavirus VP8* Recombinant Antibody (clone mAb9) (PABJ-0170) in DB
Dot Blot analysis of PABJ-0170 was performed by coating with Rotavirus A VP4 Protein, His Tag.
The secondary antibody: HRP-Goat anti-Human IgG
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
PABJ-0170-S(P) | Human Anti-Rotavirus VP8* Recombinant Antibody (clone mAb9); scFv Fragment | ELISA, Neut | Human scFv |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABJ-0170, RRID: AB_3111613)
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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