Chicken Anti-RPP30 Recombinant Antibody (clone 5C9)
CAT#: FAMAB-0006JF
Recombinant chicken monoclonal antibody specially binds to human Rpp30. This anti-Rpp30 antibody 5C9 can be potentially wildly used in the treatment of Systemic sclerosis, Raynaud's Phenomenon, systemic lupus erythematosus and poly-myositis.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, nucleoli & microtubule ends.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37578/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts and Leydig cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37578/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37578/74370_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37578/74370_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37578/74370_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37578/74370_A_5_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000148688-RPP30
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Specifications
- Immunogen
- Antigen mixture 1 (annexin XI, centromere protein B, DNA topoisomerase I, histidyl tRNA synthetase, Ro60, Rpp38 and U1A) and mixture 2 (a-fodrin, human Pop1 protein, heat shock protein B3, Ro52, Rpp30, Rrp4 and Rrp42)
- Host Species
- Chicken
- Derivation
- Chicken phage display scFv library
- Specificity
- Human Rpp30
- Species Reactivity
- Human
- Clone
- 5C9
- Applications
- WB, IF
- Related Disease
- Systemic sclerosis, Raynaud's Phenomenon, Systemic lupus erythematosus, Poly-myositis
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The RPP30 antibody has been reported in applications of Western Blot, Immunofluorescence.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone 5C9"
- CAT
- Product Name
See other products for "RPP30"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3382z | Mouse Anti-RPP30 Recombinant Antibody (clone 39H7) | WB, IP, FuncS | Mouse IgG1 |
| MOB-0806MZ | Recombinant Mouse Anti-Human RPP30 Antibody (clone 3042E6b) | WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0006JF-F(E) | Chicken Anti-RPP30 Recombinant Antibody (clone 5C9); Fab Fragment | IB, WB, ELISA, FuncS | Chicken Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0006JF-S(P) | Chicken Anti-RPP30 Recombinant Antibody (clone 5C9); scFv Fragment | IB, WB, ELISA, FuncS | Chicken scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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