Rabbit Anti-RPS6KA1 (pS380) Recombinant Antibody (clone R05-7F3)
CAT#: VS3-WK2045
This product is a rabbit recombinant antibody that is specific to phosphorylated RPS6KA1. The target of this antibody is phospho-RPS6KA1 (Ser380). The clone R05-7F3 can be used in WB analysis at a dilution of 1/500-1/1000 and IP analysis at a dilution of 1/20. The molecular weight of RPS6KA1 (pSer380) observed in WB experiments using this antibody is 90 kDa.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/21_H4_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Preleptotene spermatocytes
Staining: Low
Intensity: Moderate
Quantity: <25%
Round or early spermatids
Staining: Low
Intensity: Moderate
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (HMC-1, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117676-RPS6KA1/subcellular
❮
❯
WB
Figure 1 Rabbit Anti-RPS6KA1 (pSer380) Recombinant Antibody (clone R05-7F3) in WB.
Western blot analysis of RSK1 p90 (phospho-Ser380) in CHO-K1 lysates using anti-RPS6KA1 (pSer380) antibody, Cat# VS3-WK2045.
❮
❯
Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues Ser380 of human RSK1 p90
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Ser380 of hamster and mouse RPS6KA1
- Species Reactivity
- Hamster, Mouse
- Clone
- R05-7F3
- Applications
- WB, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IP: 1/20
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-WK2045. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "RPS6KA1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2731z | Mouse Anti-RPS6KA1 Recombinant Antibody (clone 10H12) | WB, IHC | Mouse IgG2a |
| MOB-0827MZ | Recombinant Mouse Anti-Human RPS6KA1 Antibody (clone 8C12) | ELISA | Mouse antibody |
| VS3-WK2050 | Rabbit Anti-RPS6KA1 (pT359/pS363) Recombinant Antibody (clone R33-5H-9) | WB, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS7-0425-WR794 | Mouse Anti-RPS6KA1 Monoclonal Antibody (VS7-0425-WR794) | WB, IHC-P, IF, ELISA | Mouse IgG |
| VS7-0425-WR798 | Mouse Anti-RPS6KA1 Monoclonal Antibody (clone 10B1D7A9) | IHC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3121 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KA1 Monoclonal Antibody (DS3121AB) | ICC, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY227 | AbPlus™ Anti-RPS6KA1 Magnetic Beads (CBACN-658) | IP, Protein Purification | |
| VS-0724-YC1347 | AbPlus™ Anti-RPS6KA1 Magnetic Beads (VS-0724-YC1347) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1018 | CytoStream™ Rabbit Anti-RPS6KA1 Recombinant Antibody (VS13-YC1018) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6219 | Anti-RPS6KA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6220 | Anti-Mouse RPS6KA1 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: WB, IF, FuncS
Application: FC, ADCC, CDC, Inhib
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, FuncS
Application: ELISA, IHC, WB
Application: ELISA, Block, WB, FC, IP
Application: ELISA, FC, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
