Rabbit Anti-RPS6KA5 (pS376) Recombinant Antibody (clone R84-3U-1) (CAT#: VS3-WK2056)
This product is a rabbit recombinant antibody that is specific to phosphorylated RPS6KA5. The target of this antibody is phospho-RPS6KA5 (Ser376). The clone R84-3U-1 can be used in WB analysis at a dilution of 1/500-1/1000, IF analysis at a dilution of 1/50-1/200, IP analysis at a dilution of 1/50, and IHC staining at a dilution of 1/100-1/200. The molecular weight of RPS6KA5 (pSer376) observed in WB experiments using this antibody is 80-100 kDa.
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Custom Production
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1780/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1780/if_selected.jpg
Figure 2 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells and glial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1274/ihc_selected.jpg
(Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells and glial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1274/ihc_selected.jpg
Figure 3 Cerebral cortex
(Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1274/3408_B_7_5.jpg
(Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1274/3408_B_7_5.jpg
Figure 4 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1274/3408_A_9_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1274/3408_A_9_3.jpg
Figure 5 Liver
(Cholangiocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1274/3408_A_7_4.jpg
(Cholangiocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1274/3408_A_7_4.jpg
Figure 6 Urinary bladder
(Urothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25458/62867_A_5_5.jpg
(Urothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25458/62867_A_5_5.jpg
Figure 7 Testis
(Cells in seminiferous ducts
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1274/3408_A_5_6.jpg
(Cells in seminiferous ducts
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1274/3408_A_5_6.jpg
Figure 8 RNA cell line category: Cell line enhanced (HMC-1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100784-RPS6KA5/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100784-RPS6KA5/subcellular
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Ser376 of human and rat RPS6KA5
- Species Reactivity
- Human, Rat
- Clone
- R84-3U-1
- Applications
- WB, IHC-P, ICC, IF, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/100-1/200
IF: 1/50-1/200
IP: 1/50
Target
- Alternative Names
- MSK1; RLPK; MSPK1
- Gene ID
- 9252
- UniProt ID
- O75582
- Long Name
- Ribosomal protein S6 kinase A5
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "RPS6KA5"
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Please select product type
Rabbit Monoclonal Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3124 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KA5 Monoclonal Antibody (DS3124AB) | ICC, IHC-P, IP, WB | IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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