AibGenesis™ Rabbit Anti-RPS6KA5 Recombinant Antibody (AibG-Rb-RP1743)
CAT#: VS-0326-XY568
This recombinant rabbit antibody detects RPS6KA5, a kinase regulating transcription and stress response pathways. It is reactive across species and supports studies in signal transduction. Altered activity may affect cell survival.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1780/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells and glial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1274/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1274/3408_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1274/3408_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1274/3408_A_7_4.jpg
Normal Tissue
Figure 6 Urinary bladder
Urothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25458/62867_A_5_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1274/3408_A_5_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (HMC-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100784-RPS6KA5/subcellular
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- RPS6KA5
- Species Reactivity
- Human, Mouse, Rat
- Clone
- AibG-Rb-RP1743
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Immunoprecipitation
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Buffer
- PBS, 50% glycerol and 0.05% protective protein.
- Preservative
- 0.05% Proclin 300
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1:1000-1:5000, Immunocytochemistry/Immunofluorescence: 1:200-1:1000, ELISA: 1:5000-1:20000, Immunoprecipitation: 1:50-1:200
Target
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "RPS6KA5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3124 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KA5 Monoclonal Antibody (DS3124AB) | ICC, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2348-CN | Recombinant Rabbit Anti-RPS6KA5 (phosphorylated Ser376) Monoclonal Antibody (CBACN-638) | WB, IF, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0554U | Rabbit Anti-Phospho-RPS6KA5 (S376) Recombinant Antibody (clone 2F3) | ELISA, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ179 | Rabbit Anti-RPS6KA5 Recombinant Antibody (VS3-CJ179) | WB, ICC, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK1978 | Rabbit Anti-RPS6KA5 (pS360) Recombinant Antibody (clone R03-1G8) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK2056 | Rabbit Anti-RPS6KA5 (pS376) Recombinant Antibody (clone R84-3U-1) | WB, IHC-P, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6225 | Anti-RPS6KA5 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6226 | Anti-Rat RPS6KA5 Immunohistochemistry Kit | IHC |
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