Mouse Anti-RUNX1 Recombinant Antibody (clone 6C12A5)
CAT#: VS7-0425-WR803
This product is a mouse IgG1 antibody that binds to human RUNX1. The antibody 6C12A5 recognizes specific amino acid domain of human RUNX1 (AA: 237-337). It can be used for FC assay.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4176/if_selected.jpg
Normal Tissue
Figure 2 High expression in breast
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4176/12282_B_3_4_val_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4176/12382_A_8_8.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (HEL, HMC-1, JURKAT)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000159216-RUNX1
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Specifications
- Immunogen
- Recombinant human RUNX1 (AA: 237-337) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human RUNX1
- Species Reactivity
- Human
- Clone
- 6C12A5
- Molecule Class
- Runt-Related Domain-Containing Family
- Applications
- FC
- Conjugate
- Unconjugated
- Related Disease
- Familial platelet disorder with associated myeloid malignancy (FPDMM)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- RUNX1; PEA2-Alpha B; AML1-EVI-1 Fusion Protein; CBF-Alpha-2; EVI-1; Runt-Related Transcription Factor 1; Aml1 Oncogene; PEBP2aB; Oncogene AML-1; PEBP2-Alpha B; Acute Myeloid Leukemia 1 Protein; AML1-EVI-1; SL3-3 Enhancer Factor 1 Alpha B Subunit; AMLCR1;
- Gene ID
- 861
- UniProt ID
- Q01196
- Long Name
- Runt Related Transcription Factor 1
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Acetylation; Methylation; Phosphoprotein
- Protein Refseq
- NP_001001890.1
- Function
- Activator; DNA-binding; Repressor
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "RUNX1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2922z | Mouse Anti-RUNX1 Recombinant Antibody (clone 24G12) | WB, ELISA, IF, IHC | Mouse IgG1 |
| VS3-QX990 | Mouse Anti-RUNX1 Recombinant Antibody (clone 2B5) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-XY1393 | Mouse Anti-RUNX1 Recombinant Antibody (clone 5A1) | ELISA, WB, ICC | Mouse IgG1 |
| VS7-0425-WR804 | Mouse Anti-RUNX1 Recombinant Antibody (clone 6C12B3) | WB, ELISA | Mouse IgG1 |
| VS7-0425-WR805 | Mouse Anti-RUNX1 Monoclonal Antibody (VS7-0425-WR805) | WB, IF, ICC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3142 | Hi-Affi™ Recombinant Rabbit Anti-RUNX1 Monoclonal Antibody (DS3142AB) | WB, IHC-P | IgG |
| MOR-4728 | Hi-Affi™ Recombinant Rabbit Anti-RUNX1 Monoclonal Antibody (TH242DS) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0199 | APC-A*02:01/Human EVI-1 (MILDEFYNV) MHC Tetramer | FCM | |
| MHC-CN0200 | PE-A*02:01/Human EVI-1 (MILDEFYNV) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6272 | Anti-RUNX1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6273 | Anti-Mouse RUNX1 Immunohistochemistry Kit | IHC |
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