Mouse Anti-RUNX1 Recombinant Antibody (clone 6C12A5) (CAT#: VS7-0425-WR803)
This product is a mouse IgG1 antibody that binds to human RUNX1. The antibody 6C12A5 recognizes specific amino acid domain of human RUNX1 (AA: 237-337). It can be used for FC assay.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4176/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4176/if_selected.jpg
Figure 2 High expression in breast
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4176/12282_B_3_4_val_selected.jpg
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4176/12282_B_3_4_val_selected.jpg
Figure 3 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4176/12382_A_8_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4176/12382_A_8_8.jpg
Figure 4 RNA cell line category: Cell line enhanced (HEL, HMC-1, JURKAT)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000159216-RUNX1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000159216-RUNX1
Specifications
- Immunogen
- Recombinant human RUNX1 (AA: 237-337) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human RUNX1
- Species Reactivity
- Human
- Clone
- 6C12A5
- Molecule Class
- Runt-Related Domain-Containing Family
- Applications
- FC
- Conjugate
- Unconjugated
- Related Disease
- Familial platelet disorder with associated myeloid malignancy (FPDMM)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- RUNX1; PEA2-Alpha B; AML1-EVI-1 Fusion Protein; CBF-Alpha-2; EVI-1; Runt-Related Transcription Factor 1; Aml1 Oncogene; PEBP2aB; Oncogene AML-1; PEBP2-Alpha B; Acute Myeloid Leukemia 1 Protein; AML1-EVI-1; SL3-3 Enhancer Factor 1 Alpha B Subunit; AMLCR1;
- Gene ID
- 861
- UniProt ID
- Q01196
- Long Name
- Runt Related Transcription Factor 1
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Acetylation; Methylation; Phosphoprotein
- Protein Refseq
- NP_001001890.1
- Function
- Activator; DNA-binding; Repressor
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2922z | Mouse Anti-RUNX1 Recombinant Antibody (clone 24G12) | WB, ELISA, IF, IHC | Mouse IgG1 |
| MOB-2284CT | Recombinant Mouse anti-Human RUNX1 Monoclonal antibody (FQ2566Z) | ICC/IF, WB, IHC-P, IHC-Fr | |
| VS3-QX990 | Mouse Anti-RUNX1 Recombinant Antibody (clone 2B5) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-WK840 | Rabbit Anti-RUNX1 Recombinant Antibody (VS3-WK840) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS7-0425-WR804 | Mouse Anti-RUNX1 Recombinant Antibody (clone 6C12B3) | WB, ELISA | Mouse IgG1 |
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