CytoStream™ Rabbit Anti-S100A8 Recombinant Antibody (VS13-YC1029) (CAT#: VS13-YC1029)
The CytoStream™ Rabbit Anti-S100A8 Recombinant Antibody is specific for human S100A8. S100A8 encodes a protein linked to processes like kinase inhibition and cytokine function, with altered expression associated with cystic fibrosis, existing in transcript variants. The anti-S100A8 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/1000).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HaCaT
(Immunofluorescent staining of human cell line HaCaT shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24372/1573_E7_2_selected.jpg
(Immunofluorescent staining of human cell line HaCaT shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24372/1573_E7_2_selected.jpg
Figure 2 Bone marrow
(Hematopoietic cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24372/55060_B_6_4.jpg
(Hematopoietic cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24372/55060_B_6_4.jpg
Figure 3 Lymph node
(Non-germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24372/55060_A_8_8.jpg
(Non-germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24372/55060_A_8_8.jpg
Figure 4 RNA cell line category: Group enriched (hTCEpi, SK-BR-3, THP-1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143546-S100A8
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143546-S100A8
Figure 4 RNA cell line category: Group enriched (hTCEpi, SK-BR-3, THP-1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143546-S100A8
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143546-S100A8
Specifications
- Immunogen
- Recombinant human S100A8, aa 1-93.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human S100A8
- Species Reactivity
- Human
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Conjugate
- NONE
- Related Disease
- Cystic fibrosis
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 40% Glycerol and 0.1% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/2000-1/5000
Immunohistochemistry-paraffin (IHC-P): 1/10000
Immunofluorescence (IF-cell): 1/50000
Flow cytometry (FC): 1/1000
Immunofluorescence (IF-tissue): 1/1000-1/3000
Immunoprecipitation (IP): 1-2 μg per 1x 10⁶ cells
Target
- Alternative Names
- S100 Calcium Binding Protein A8; Migration Inhibitory Factor-Related Protein 8; Leukocyte L1 Complex Light Chain; Urinary Stone Protein Band A; Calprotectin L1L Subunit; Cystic Fibrosis Antigen; MRP8; CAGA; CFAG; P8; S100 Calcium-Binding Protein A8 (Calgranulin A); S100 Calcium Binding Protein A8 (Calgranulin A); S100 Calcium-Binding Protein A8;
- Gene ID
- 6279
- UniProt ID
- P05109
- Long Name
- S100 Calcium Binding Protein A8
- Cellular Localization
- Secreted; Cytoplasm; Cytoskeleton; Cell membrane
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1209z | Mouse Anti-S100A8 Recombinant Antibody (clone 28H11) | WB, ELISA | Mouse IgG1, κ |
| MOB-0919MZ | Recombinant Mouse Anti-Human S100A8 Antibody (clone 3D6/5) | ELISA, IHC-P, WB | Mouse antibody |
| MRO-2043-CN | Rabbit Anti-S100A8 Polyclonal Antibody (MRO-2043-CN) | WB, IF, IHC, FC | Rabbit IgG |
| FAMAB-1535CQ | Human Anti-S100A8 Recombinant Antibody (clone 5.5) | IP, WB, ELISA, FC, IHC | Human IgG1 |
| ZG-0169U | Mouse Anti-S100A8 Recombinant Antibody (clone 1A1C1) | ELISA, IHC | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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