CytoStream™ Rabbit Anti-S100A8 Recombinant Antibody (VS13-YC1029)
CAT#: VS13-YC1029
The CytoStream™ Rabbit Anti-S100A8 Recombinant Antibody is specific for human S100A8. S100A8 encodes a protein linked to processes like kinase inhibition and cytokine function, with altered expression associated with cystic fibrosis, existing in transcript variants. The anti-S100A8 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/1000).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24372/1573_E7_2_selected.jpg
Normal Tissue
Figure 2 Bone marrow
Hematopoietic cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24372/55060_B_6_4.jpg
Normal Tissue
Figure 3 Lymph node
Non-germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24372/55060_A_8_8.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (hTCEpi, SK-BR-3, THP-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143546-S100A8
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Specifications
- Immunogen
- Recombinant human S100A8, aa 1-93.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human S100A8
- Species Reactivity
- Human
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Conjugate
- NONE
- Related Disease
- Cystic fibrosis
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 40% Glycerol and 0.1% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/2000-1/5000
Immunohistochemistry-paraffin (IHC-P): 1/10000
Immunofluorescence (IF-cell): 1/50000
Flow cytometry (FC): 1/1000
Immunofluorescence (IF-tissue): 1/1000-1/3000
Immunoprecipitation (IP): 1-2 μg per 1x 10⁶ cells
Target
- Alternative Names
- S100 Calcium Binding Protein A8; Migration Inhibitory Factor-Related Protein 8; Leukocyte L1 Complex Light Chain; Urinary Stone Protein Band A; Calprotectin L1L Subunit; Cystic Fibrosis Antigen; MRP8; CAGA; CFAG; P8; S100 Calcium-Binding Protein A8 (Calgranulin A); S100 Calcium Binding Protein A8 (Calgranulin A); S100 Calcium-Binding Protein A8;
- Gene ID
- 6279
- UniProt ID
- P05109
- Long Name
- S100 Calcium Binding Protein A8
- Cellular Localization
- Secreted; Cytoplasm; Cytoskeleton; Cell membrane
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "S100A8"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0919MZ | Recombinant Mouse Anti-Human S100A8 Antibody (clone 3D6/5) | ELISA, IHC-P, WB | Mouse antibody |
| HPAB-0501-CN | Mouse Anti-S100A8 Recombinant Antibody (HPAB-0501-CN) | ELISA, FC | Mouse IgG |
| MRO-2043-CN | Rabbit Anti-S100A8 Polyclonal Antibody (MRO-2043-CN) | WB, IF, IHC, FC | Rabbit IgG |
| FAMAB-1535CQ | Human Anti-S100A8 Recombinant Antibody (clone 5.5) | IP, WB, ELISA, FC, IHC | Human IgG1 |
| ZG-0169U | Mouse Anti-S100A8 Recombinant Antibody (clone 1A1C1) | ELISA, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3158 | Hi-Affi™ Recombinant Rabbit Anti-S100A8 Monoclonal Antibody (DS3158AB) | ICC, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0501-CN-S(P) | Mouse Anti-S100A8 Recombinant Antibody; scFv Fragment (HPAB-0501-CN-S(P)) | ELISA, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0501-CN-F(E) | Mouse Anti-S100A8 Recombinant Antibody; Fab Fragment (HPAB-0501-CN-F(E)) | ELISA, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1127 | AbPlus™ Anti-S100A8 Magnetic Beads (VS-0724-YC1127) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1994 | Anti-S100A8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6313 | Anti-Human S100A8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6314 | Anti-Mouse S100A8 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC284 | Recombinant Anti-S100A8 Eliminating Antibody, pH-Sensitive (5.5 var.) | Antigen-Sweeping In Vivo. | Human IgG1 |
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