Rabbit Anti-SATB2 Recombinant Antibody (clone R02-1H5)
CAT#: VS3-FY1624
This anti-SATB2 antibody detected positive in western blot analysis of SATB2 in human and rat brain lysates. The recombinant monoclonal antibody is meticulously crafted to specifically target human and rat SATB2. The R02-1H5 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000).
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29543/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67751/152042_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67751/152042_A_9_3.jpg
Normal Tissue
Figure 4 Rectum
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67751/152042_A_4_3.jpg
Normal Tissue
Figure 5 Kidney
Proximal tubules (cell body)
Staining: Low
Intensity: Moderate
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67751/152042_A_8_5.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HEL, K-562, THP-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000119042-SATB2/subcellular
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WB
Figure 1 Anti-SATB2 Antibody (VS3-FY1624) in WB.
The SATB2 expression in rat Brain lysates were detected using the anti-SATB2 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human SATB2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat SATB2
- Species Reactivity
- Human, Rat
- Clone
- R02-1H5
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 83 kDa, while the observed molecular weight stands at 83 kDa.
- Related Disease
- Glass Syndrome and Cleft Palate, Isolated
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- SATB Homeobox 2; Special AT-Rich Sequence-Binding Protein 2; SATB Family Member 2; DNA-Binding Protein SATB2; KIAA1034; GLSS
- Gene ID
- 23314
- UniProt ID
- Q9UPW6
- Sequence Similarities
- Belongs to the CUT homeobox family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Sumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity.
- Protein Refseq
- NP_001165980.1; NP_001165988.1; NP_056080.1
- Function
- Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SATB2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-888 | Recombinant Anti-human SATB2 Antibody | WB, ELISA, IHC, FuncS | IgG |
| MOB-2453CT | Recombinant Mouse anti-Human SATB2 Monoclonal antibody (nBcdbn) | IP, ICC, IF, IHC-FoFr, WB, IHC-Fr, IHC-P | |
| VS3-QX1000 | Mouse Anti-SATB2 Recombinant Antibody (clone 4G03) | WB, IP, IF | Mouse IgG1 |
| VS7-0425-WR828 | Mouse Anti-SATB2 Recombinant Antibody (clone 7B2E11) | IHC, FC | Mouse IgG1 |
| VS7-0425-WR829 | Mouse Anti-SATB2 Recombinant Antibody (clone 7B2C4) | IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-888-F(E) | Recombinant Anti-human SATB2 Antibody Fab Fragment | ELISA, WB, FuncS | Fab |
| MHH-888-F(E) | Recombinant Human Anti-human SATB2 Antibody Fab Fragment | IP, Neut, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-888-S(P) | Recombinant Anti-human SATB2 Antibody scFv Fragment | WB, Neut, FuncS | scFv |
| MHH-888-S(P) | Recombinant Human Anti-human SATB2 Antibody scFv Fragment | WB, FC, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3175 | Hi-Affi™ Recombinant Rabbit Anti-SATB2 Monoclonal Antibody (DS3175AB) | WB, IP, IHC-P, ICC, IF, IHC-Fr | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1038 | CytoStream™ Rabbit Anti-SATB2 Recombinant Antibody (VS13-YC1038) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6356 | Anti-SATB2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6357 | Anti-Mouse SATB2 Immunohistochemistry Kit | IHC |
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