Rabbit Anti-SDHB Recombinant Antibody (clone 7D12) (CAT#: ZG-0558U)
Rabbit Anti-SDHB Antibody (clone 7D12) displayed high specificity against the SDHB protein of Human, Mouse and Rat.
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WB
Figure 1 Rabbit Anti-SDHB Antibody (ZG-0558U) in WB
Western Blot
Positive WB detected in: 293 whole cell lysate, K562 whole cell lysate, NIH/3T3 whole cell lysate, PC-3 whole cell lysate, Rat heart tissue, Mouse heart tissue, Mouse liver tissue
All lanes: SDHB antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDa
IHC
Figure 2 Rabbit Anti-SDHB Antibody (ZG-0558U) in IHC
IHC image of ZG-0558U diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC
Figure 3 Rabbit Anti-SDHB Antibody (ZG-0558U) in IHC
IHC image of ZG-0558U diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
FC
Figure 4 Rabbit Anti-SDHB Antibody (ZG-0558U) in FC
Overlay histogram showing 293 cells stained with ZG-0558U (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10, 000 events was performed.
Specifications
- Immunogen
- A synthesized peptide derived from human SDHB
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SDHB
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 7D12
- Applications
- ELISA, WB, IHC, FC
- Conjugate
- Unconjugated
- Related Disease
- Paragangliomas 4 and Pheochromocytoma.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Flow Cytometry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200, FC:1:20-1:200
Target
- Alternative Names
- Succinate dehydrogenase [ubiquinone] iron-sulfur subunit; mitochondrial; EC 1.3.5.1; Iron-sulfur subunit of complex II; Ip; SDHB; SDH SDH1
- Gene ID
- 6390
- UniProt ID
- P21912
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3584z | Mouse Anti-SDHB Recombinant Antibody (clone 41B8) | WB, IP, IF, ELISA | Mouse IgG1, κ |
| MOB-2470CT | Recombinant Mouse anti-Cow SDHB Monoclonal antibody (0H20) | FC, IHC-Fr, ICC, IHC-P, WB, ICC/IF | |
| MRO-1373-CN | Recombinant Rabbit Anti-SDHB Monoclonal Antibody (JU02-33) | WB, IHC, FC, IP | Rabbit IgG |
| VS7-0425-WR836 | Mouse Anti-SDHB Recombinant Antibody (clone 2B2B1) | IHC, FC | Mouse IgG1 |
| VS7-0425-WR837 | Mouse Anti-SDHB Recombinant Antibody (clone 2E8B11) | IHC, FC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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