Recombinant Rabbit Anti-SEC23A Monoclonal Antibody (JU37-48)
CAT#: MRO-1374-CN
This antibody is a rabbit monoclonal antibody that is capable of binding to SEC23A.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43806/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69974/159502_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69974/159502_A_7_3.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69974/159502_A_6_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69974/159502_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (Hep G2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100934-SEC23A
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Specifications
- Immunogen
- Recombinant protein within human SEC23 aa 300-500
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human
- Species Reactivity
- Human, Mouse
- Clone
- JU37-48
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunofluorescence; Immunohistochemistry; Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Format
- Liquid
- Buffer
- 1*TBS, pH 7.4, 1% BSA, 40% Glycerol
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
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Customer Review
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WB Star
I used this recombinant rabbit anti - SEC23A monoclonal antibody in a Western blot, and it was a star performer. The protein A affinity purification really showed; I got sharp, clear bands for SEC23A. This made it easy to analyze the expression levels in different tissue samples. Considering its use in Western blot for studying SEC23A in various tissues like the colon and testis, and its role in protein transport mechanisms, this antibody has been a great addition to my research toolkit.
IF Excellent
For immunofluorescence experiments to study SEC23A localization in cell lines, this antibody was excellent. After optimizing the staining conditions, I could clearly see the protein in the nucleoplasm and vesicles as expected. The high sensitivity of the antibody allowed for precise visualization. Given its application in immunofluorescence and its importance in understanding the subcellular location of SEC23A in cell - based research, I'm very satisfied with its performance.
IHC Reliable
I used this antibody in an immunohistochemistry experiment on brain tissue to study SEC23A expression in neuronal cells. The results were reliable. After following the proper steps like antigen retrieval and optimizing the antibody dilution, I got clear staining in the neuronal cells. Considering its use in immunohistochemistry for studying SEC23A in specific cell types within tissues, it has proven to be a trustworthy antibody.
Q&As
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I'm interested in how SEC23A functions in the protein transport mechanism. How can this antibody help in my research?
A: This anti - SEC23A antibody can be a valuable tool. In immunoprecipitation experiments, you can use it to pull down SEC23A along with its interacting proteins. By identifying these interacting partners, you can gain insights into the protein-protein interactions involved in the protein transport process. For example, if SEC23A forms a complex with other components of the COPII coat complex during protein transport from the endoplasmic reticulum to the Golgi apparatus, immunoprecipitation with this antibody can help isolate and study this complex. Additionally, in immunohistochemistry, localizing SEC23A in tissues can show where in the cell or tissue the protein transport activity is occurring.
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I'm conducting a comparative study on SEC23A expression in human and mouse tissues. How does the species reactivity of this antibody ensure reliable cross - species analysis?
A: The antibody's reactivity with both human and mouse SEC23A is based on conserved epitopes between the two species. When performing immunoassays such as immunohistochemistry or Western blot on tissues from human and mouse, you can use the same antibody. However, it's important to note that there may be minor differences in the protein structure or expression levels. You should optimize experimental conditions like antibody dilution and incubation times for each species. This way, you can account for any species - specific variations and ensure reliable cross - species analysis of SEC23A expression, which is useful for understanding the conserved and divergent functions of SEC23A in different organisms.
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I want to use this antibody in an immunohistochemistry experiment on brain tissue to study SEC23A expression in neuronal cells. What are the key steps to ensure accurate results?
A: First, proper tissue fixation is crucial. Use a fixative that preserves the tissue architecture and SEC23A antigenicity, such as formalin. After fixation, perform antigen retrieval, which can enhance the antibody's access to the target antigen. Since the antibody is specific for SEC23A and has high sensitivity, optimize the antibody dilution. In brain tissue, neuronal cells have a complex structure, so careful washing between steps is necessary to reduce background staining. Also, use appropriate positive and negative controls. A positive control could be a known SEC23A - expressing region in the brain, and a negative control could be tissue incubated without the primary antibody.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "SEC23A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3199 | Hi-Affi™ Recombinant Rabbit Anti-SEC23A Monoclonal Antibody (DS3199AB) | WB, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1960 | PE-A2/Human SEC23A (SLLPPTALVGL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6424 | Anti-SEC23A Immunohistochemistry Kit | IHC |
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