Rabbit Anti-SEPT7 Recombinant Antibody (clone R09-6A1)
CAT#: VS3-FY1880
Clone R09-6A1 is a rabbit recombinant antibody that has a high affinity for human SEPT7 protein, a calculated weight of 51 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R09-6A1 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunofluorescence (dilution range 1:50-1:200).
Specifications
- Immunogen
- Recombinant human SEPT7 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat SEPT7
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R09-6A1
- Applications
- Western Blot, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 51 kDa, while the observed molecular weight stands at 51 kDa.
- Related Disease
- Amyotrophic Neuralgia and Brachial Plexus Neuropathy
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Septin 7; CDC10 Protein Homolog; CDC10; CDC10 (Cell Division Cycle 10, S. Cerevisiae, Homolog); CDC10 Cell Division Cycle 10 Homolog (S. Cerevisiae); Septin 7 Variant 4
- Gene ID
- 989
- UniProt ID
- Q16181
- Sequence Similarities
- Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.
- Cellular Localization
- Cell projection, Centromere, Chromosome, Cilium, Cytoplasm
- Post Translation Modifications
- Ubiquitination at Lys89, Lys298, and Lys313 (NX_Q16181 [NX_Q16181-1])
Modification sites at PhosphoSitePlus (Q16181)
Glycosylation from GlyGen (Q16181) 6 sites, 1 O-linked glycan (6 sites)
- Protein Refseq
- XP_006715869.1; XP_011513958.1; XP_011513963.1; XP_047277051.1; XP_047277052.1
- Function
- Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SEPT7"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2032 | Anti-SEPT7 Immunohistochemistry Kit | IHC |
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