Human Anti-SIGLEC8 Recombinant Antibody (clone 2C4)
CAT#: VS-0624-FY31
This is a humanized and non-fucosylated anti-Siglec-8 IgG1 antibody. The clone engages with the N-terminal and the B'-C loop at the V domain.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_A_8_5.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enriched (HMC-1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105366-SIGLEC8
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Specifications
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- Human SIGLEC8
- Species Reactivity
- Human
- Clone
- 2C4
- Applications
- Flow Cytometry
- Related Disease
- Eosinophilic Gastritis and Chronic Inducible Urticaria
Product Property
- Clonality
- Monoclonal Antibody
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Sialic Acid Binding Ig Like Lectin 8; Sialoadhesin Family Member 2; SIGLEC-8; SAF-2; SAF2; Sialic Acid Binding Ig-Like Lectin 8; Sialic Acid-Binding Ig-Like Lectin 8; SIGLEC8L; CDw329
- Gene ID
- 27181
- UniProt ID
- Q9NYZ4
- Long Name
- Sialic Acid Binding Ig Like Lectin 8
- Sequence Similarities
- Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
- Cellular Localization
- Membrane
- Post Translation Modifications
- Glycosylation at Asn249, Asn172, and Asn267 (NX_Q9NYZ4 [NX_Q9NYZ4-1])
Modification sites at PhosphoSitePlus (Q9NYZ4)
Glycosylation from GlyConnect SIGL8_HUMAN (570)
Glycosylation from GlyGen (Q9NYZ4) 4 sites, 27 N-linked glycans (1 site)
- Protein Refseq
- XP_011525036.1; XP_054176546.1; NP_001350477.1; NP_055257.2
- Function
- Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells (PubMed:10856141, 10625619).
Preferentially binds to alpha-2,3-linked sialic acid.
Also binds to alpha-2,6-linked sialic acid.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SIGLEC8"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-452CT | Human Anti-SIGLEC8 Recombinant Antibody (TAB-452CT) | WB, ELISA, FC | Humanized antibody |
| TAB-452CT-S(P) | Human Anti-SIGLEC8 Recombinant Antibody; scFv Fragment (TAB-452CT-S(P)) | WB, ELISA, FC | Human scFv |
| TAB-452CT-F(E) | Human Anti-SIGLEC8 Recombinant Antibody; Fab Fragment (TAB-452CT-F(E)) | WB, ELISA, FC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1378CT | Recombinant Mouse anti-Human SIGLEC8 Monoclonal antibody (EML1447) | WB | |
| HPAB-S0136-YC | Mouse Anti-SIGLEC8 Recombinant Antibody (HPAB-S0136-YC) | ELISA, FC | Mouse IgG2, κ |
| VS3-FY2872 | Mouse Anti-SIGLEC8 Recombinant Antibody (VS3-FY2872) | WB, IHC, FC, ELISA | Mouse IgG |
| VS7-0425-WR853 | Mouse Anti-SIGLEC8 Recombinant Antibody (clone 1A5B9) | WB, IHC, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-S0136-YC-F(E) | Mouse Anti-SIGLEC8 Recombinant Antibody; Fab Fragment (HPAB-S0136-YC-F(E)) | ELISA, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-S0136-YC-S(P) | Mouse Anti-SIGLEC8 Recombinant Antibody; scFv Fragment (HPAB-S0136-YC-S(P)) | ELISA, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC446 | Recombinant Anti-SIGLEC8 Vesicular Antibody, EV Displayed (VS-0425-YC446) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6586 | Anti-SIGLEC8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6587 | Anti-Human SIGLEC8 Immunohistochemistry Kit | IHC |
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