Mouse Anti-SKP2 Monoclonal Antibody (clone 6G9D10)
CAT#: VS7-0425-WR868
This product is a mouse IgG1 antibody that binds to human SKP2. The antibody 6G9D10 recognizes specific amino acid domain of SKP2 (aa1-130). It can be used for WB and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm, nucleoli & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51196/if_selected.jpg
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Specifications
- Immunogen
- Recombinant SKP2 (aa1-130) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human SKP2
- Species Reactivity
- Human
- Clone
- 6G9D10
- Molecule Class
- F-Box Protein
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- p45; FBL1; FLB1; FBXL1
- Gene ID
- 6502
- UniProt ID
- Q13309
- Long Name
- S-phase kinase associated protein 2
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Acetylation; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001230049.1
- Function
- Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription (PubMed:11931757, PubMed:12435635, PubMed:12769844, PubMed:12840033, PubMed:15342634, PubMed:15668399, PubMed:15949444, PubMed:16103164, PubMed:16262255, PubMed:16581786, PubMed:16951159, PubMed:17908926, PubMed:17962192, PubMed:22464731, PubMed:22770219, PubMed:32267835).; Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition (By similarity).; Degradation of CDKN1B/p27kip also requires CKS1 (By similarity).; Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, NBN, FOXO1, UBP43, YTHDF2, and probably MYC, TOB1 and TAL1 (PubMed:11931757, PubMed:12435635, PubMed:12769844, PubMed:12840033, PubMed:15342634, PubMed:15668399, PubMed:15949444, PubMed:16103164, PubMed:16581786, PubMed:16951159, PubMed:17908926, PubMed:17962192, PubMed:22464731, PubMed:32267835).; Degradation of TAL1 also requires STUB1 (PubMed:17962192).; Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2 (PubMed:16262255).; Promotes ubiquitination and destruction of CDH1 in a CK1-dependent manner, thereby regulating cell migration (PubMed:22770219).; Following phosphorylation in response to DNA damage, mediates 'Lys-63'-linked ubiquitination of NBN, promoting ATM recruitment to DNA damage sites and DNA repair via homologous recombination (PubMed:22464731).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SKP2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3275 | Hi-Affi™ Recombinant Rabbit Anti-SKP2 Monoclonal Antibody (DS3275AB) | WB, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-091R | Mouse Anti-SKP2 Recombinant Antibody (ZG-091R) | WB, ELISA | Mouse IgG |
| ZG-0126U | Rabbit Anti-SKP2 Recombinant Antibody (clone 6D4) | ELISA, IHC, IF | Rabbit IgG |
| ZG-0562U | Rabbit Anti-SKP2 Recombinant Antibody (clone 3C11) | ELISA, IF, FC | Rabbit IgG |
| VS3-QX1016 | Mouse Anti-SKP2 Recombinant Antibody (clone 4D8-B9-F3) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-FY1343 | Recombinant Rabbit Anti-SKP2 Antibody (clone R08-0A2) | WB, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY483 | Mouse Anti-NHP SKP2 Recombinant Antibody (clone SKP2-8D9) | WB, IF, IP, ELISA | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2080 | Anti-SKP2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6616 | Anti-Human SKP2 Immunohistochemistry Kit | IHC |
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