Rabbit Anti-SKP2 Recombinant Antibody (clone 6D4) (CAT#: ZG-0126U)
Rabbit anti-SKP2 antibody (clone 6D4) displayed high specificity against the SKP2 protein of Human.
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Custom Production
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IHC
Figure 1 Rabbit Anti-SKP2 Antibody (ZG-0126U) in IHC
IHC image of ZG-0126U diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IF
Figure 2 Rabbit Anti-SKP2 Antibody (ZG-0126U) in IF
Immunofluorescence staining of Hela Cells with ZG-0126U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human SKP2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SKP2
- Species Reactivity
- Human
- Clone
- 6D4
- Applications
- ELISA, IHC, IF
- Conjugate
- Unconjugated
- Related Disease
- Lung Cancer and Myxofibrosarcoma
Product Property
- Purification
- Affinity Purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Immunofluorescence. Specific conditions should be optimized by the end user.
IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- p45; FBL1; FLB1; FBXL1; S-phase kinase-associated protein 2; CDK2/cyclin A-associated protein p45; F-box/LRR-repeat protein 1; S-phase kinase-associated protein 2, E3 ubiquitin protein ligase; p45skp2
- Gene ID
- 6502
- UniProt ID
- Q13309
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1784MZ | Recombinant Mouse Anti-Human SKP2 Antibody (clone nBcdbn79998) | WB | Mouse antibody |
| ZG-091R | Mouse Anti-SKP2 Recombinant Antibody (ZG-091R) | WB, ELISA | Mouse IgG |
| ZG-0562U | Rabbit Anti-SKP2 Recombinant Antibody (clone 3C11) | ELISA, IF, FC | Rabbit IgG |
| VS3-QX1016 | Mouse Anti-SKP2 Recombinant Antibody (clone 4D8-B9-F3) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS7-0425-WR868 | Mouse Anti-SKP2 Monoclonal Antibody (clone 6G9D10) | WB, ELISA | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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