Rabbit Anti-SNAP25 Recombinant Antibody (VS3-CJ1069)
CAT#: VS3-CJ1069
This product is a rabbit antibody that recognizes human, mouse, and rat SNAP25.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71013/1916_E10_26_cr5c99f28cf12b4_selected.jpg
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Specifications
- Immunogen
- Peptide
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat SNAP25
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IP
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 0.5% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation.
Target
- Alternative Names
- SUP; RIC4; SEC9; SNAP; CMS18; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
- Gene ID
- 6616
- UniProt ID
- P60880
- Sequence Similarities
- Belongs to the SNAP-25 family.
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane, Synapse, Synaptosome
- Post Translation Modifications
- Palmitoylated (PubMed:28757145).
Cys-85 appears to be the main site, and palmitoylation is required for membrane association (By similarity).
(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type A (BoNT/A, botA) which hydrolyzes the 197-Gln-|-Arg-198 bond and inhibits neurotransmitter release (PubMed:15592454, PubMed:9886085).
(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type C (BoNT/C) which hydrolyzes the 198-Arg-|-Ala-199 bond and inhibits neurotransmitter release (PubMed:9886085, PubMed:17718519).
C.botulinum type C only rarely infects humans.
(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type E (BoNT/E) which hydrolyzes the 180-Arg-|-Ile-181 bond and inhibits neurotransmitter release (PubMed:9886085).
- Protein Refseq
- NP_001309831.1; NP_001309832.1; NP_001309833.1
- Function
- t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SNAP25"
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|---|---|---|---|
| MOB-912-F(E) | Recombinant Anti-SNAP25 Antibody Fab Fragment | RIA, IF, FuncS | Fab |
| MHH-912-F(E) | Recombinant Human Anti-SNAP25 Antibody Fab Fragment | WB, ELISA, IHC, FuncS | Fab |
| HPAB-0019-CN-F(E) | Mouse Anti-SNAP25 Recombinant Antibody; Fab Fragment (HPAB-0019-CN-F(E)) | ELISA, ICC, IP | Mouse Fab |
| HPAB-0020-CN-F(E) | Mouse Anti-SNAP25 Recombinant Antibody; Fab Fragment (HPAB-0020-CN-F(E)) | ELISA, ICC, IP | Mouse Fab |
| HPAB-0021-CN-F(E) | Mouse Anti-SNAP25 Recombinant Antibody; Fab Fragment (HPAB-0021-CN-F(E)) | ELISA, ICC, IP | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-912-S(P) | Recombinant Anti-SNAP25 Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
| MHH-912-S(P) | Recombinant Human Anti-SNAP25 Antibody scFv Fragment | WB, IHC, FuncS | scFv |
| HPAB-0019-CN-S(P) | Mouse Anti-SNAP25 Recombinant Antibody; scFv Fragment (HPAB-0019-CN-S(P)) | ELISA, ICC, IP | Mouse scFv |
| HPAB-0546-FY-S(P) | Human Anti-SNAP25 Recombinant Antibody; scFv Fragment (HPAB-0546-FY-S(P)) | WB, IHC | Humanized scFv |
| HPAB-0547-FY-S(P) | Mouse Anti-SNAP25 Recombinant Antibody; scFv Fragment (HPAB-0547-FY-S(P)) | WB, IHC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1896CT | Recombinant Mouse anti-Human SNAP25 Monoclonal antibody (TQ23) | ELISA, IHC-P, WB | |
| HPAB-0546-FY | Human Anti-SNAP25 Recombinant Antibody (HPAB-0546-FY) | WB, IHC | Humanized IgG1 |
| FAMAB-1551CQ | Mouse Anti-SNAP-25 Recombinant Antibody (clone SP12) | WB, ELISA, IF, IHC | Mouse IgG1 |
| ZG-0756F | Mouse Anti-SNAP25 Recombinant Antibody (clone B-29-F9) | WB, IHC-P | Mouse IgG1 |
| ZG-0899F | Mouse Anti-SNAP25 Recombinant Antibody (clone S-7B8) | WB, IHC-P | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3351 | Hi-Affi™ Recombinant Rabbit Anti-SNAP25 Monoclonal Antibody (DS3351AB) | WB, IP, ICC, IF | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0020-CN | Mouse Anti-SNAP25 Recombinant Antibody (HPAB-0020-CN) | ELISA, ICC, IP | Mouse IgG, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY243 | AbPlus™ Anti-SNAP25 Magnetic Beads (VS-0424-XY243) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY488 | Mouse Anti-NHP SNAP25 Recombinant Antibody (clone S-7B8) | IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2128 | Anti-SNAP25 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6768 | Anti-Mouse SNAP25 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6767 | Anti-Human SNAP25 Immunohistochemistry Kit | IHC |
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