Rabbit Anti-SNAP25 Recombinant Antibody (VS3-CJ1069) (CAT#: VS3-CJ1069)
This product is a rabbit antibody that recognizes human, mouse, and rat SNAP25.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71013/1916_E10_26_cr5c99f28cf12b4_selected.jpg
(Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/71013/1916_E10_26_cr5c99f28cf12b4_selected.jpg
Figure 2 Cerebral cortex
(Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/360/2293_B_8_5.jpg
(Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/360/2293_B_8_5.jpg
Figure 3 RNA cell line category: Cell line enhanced (Karpas-707, SCLC-21H, SH-SY5Y, U-87 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132639-SNAP25/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132639-SNAP25/subcellular
Specifications
- Immunogen
- Peptide
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat SNAP25
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IP
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 0.5% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation.
Target
- Alternative Names
- SUP; RIC4; SEC9; SNAP; CMS18; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
- Gene ID
- 6616
- UniProt ID
- P60880
- Sequence Similarities
- Belongs to the SNAP-25 family.
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane, Synapse, Synaptosome
- Post Translation Modifications
- Palmitoylated (PubMed:28757145).
Cys-85 appears to be the main site, and palmitoylation is required for membrane association (By similarity).
(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type A (BoNT/A, botA) which hydrolyzes the 197-Gln-|-Arg-198 bond and inhibits neurotransmitter release (PubMed:15592454, PubMed:9886085).
(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type C (BoNT/C) which hydrolyzes the 198-Arg-|-Ala-199 bond and inhibits neurotransmitter release (PubMed:9886085, PubMed:17718519).
C.botulinum type C only rarely infects humans.
(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type E (BoNT/E) which hydrolyzes the 180-Arg-|-Ile-181 bond and inhibits neurotransmitter release (PubMed:9886085).
- Protein Refseq
- NP_001309831.1; NP_001309832.1; NP_001309833.1
- Function
- t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "SNAP25"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0211MZ | Recombinant Mouse Anti-SNAP-25 (amino acid 183-197) cleaved Antibody (clone C318M) | ELISA, IHC | Mouse antibody |
| MOB-1896CT | Recombinant Mouse anti-Human SNAP25 Monoclonal antibody (TQ23) | ELISA, IHC-P, WB | |
| HPAB-0546-FY | Human Anti-SNAP25 Recombinant Antibody (HPAB-0546-FY) | WB, IHC | Humanized IgG1 |
| HPAB-0547-FY | Mouse Anti-SNAP25 Recombinant Antibody (HPAB-0547-FY) | WB, IHC | Mouse IgG2a |
| FAMAB-1551CQ | Mouse Anti-SNAP-25 Recombinant Antibody (clone SP12) | WB, ELISA, IF, IHC | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3351 | Hi-Affi™ Recombinant Rabbit Anti-SNAP25 Monoclonal Antibody (DS3351AB) | WB, IP, ICC/IF | IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0019-CN-S(P) | Mouse Anti-SNAP25 Recombinant Antibody; scFv Fragment (HPAB-0019-CN-S(P)) | ELISA, ICC, IP | Mouse scFv |
| HPAB-0020-CN-S(P) | Mouse Anti-SNAP25 Recombinant Antibody; scFv Fragment (HPAB-0020-CN-S(P)) | ELISA, ICC, IP | Mouse scFv |
| HPAB-0021-CN-S(P) | Mouse Anti-SNAP25 Recombinant Antibody; scFv Fragment (HPAB-0021-CN-S(P)) | ELISA, ICC, IP | Mouse scFv |
| HPAB-0022-CN-S(P) | Mouse Anti-SNAP25 Recombinant Antibody; scFv Fragment (HPAB-0022-CN-S(P)) | ELISA, ICC, IP | Mouse scFv |
| HPAB-0023-CN-S(P) | Mouse Anti-SNAP25 Recombinant Antibody; scFv Fragment (HPAB-0023-CN-S(P)) | ELISA, ICC, IHC | Mouse scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0019-CN-F(E) | Mouse Anti-SNAP25 Recombinant Antibody; Fab Fragment (HPAB-0019-CN-F(E)) | ELISA, ICC, IP | Mouse Fab |
| HPAB-0020-CN-F(E) | Mouse Anti-SNAP25 Recombinant Antibody; Fab Fragment (HPAB-0020-CN-F(E)) | ELISA, ICC, IP | Mouse Fab |
| HPAB-0021-CN-F(E) | Mouse Anti-SNAP25 Recombinant Antibody; Fab Fragment (HPAB-0021-CN-F(E)) | ELISA, ICC, IP | Mouse Fab |
| HPAB-0022-CN-F(E) | Mouse Anti-SNAP25 Recombinant Antibody; Fab Fragment (HPAB-0022-CN-F(E)) | ELISA, ICC, IP | Mouse Fab |
| HPAB-0023-CN-F(E) | Mouse Anti-SNAP25 Recombinant Antibody; Fab Fragment (HPAB-0023-CN-F(E)) | ELISA, ICC, IHC | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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