AbPlus™ Anti-SOD1 Magnetic Beads (VS-0724-YC1461)
CAT#: VS-0724-YC1461
The AbPlus Anti-SOD1 Magnetic Beads (VS-0724-YC1461) is an innovative affinity resin which is bound with anti-SOD1 specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of SOD1 protein under native and denaturing conditions.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in glial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8670/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/30_D2_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/29_D2_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/31_D2_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/4148_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/4148_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/4148_A_8_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/4148_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/4148_A_4_6.jpg
Normal Tissue
Figure 10 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/4148_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000142168-SOD1
❮
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-SOD1 specific antibody
- Target
- SOD1
- Immunogen
- Recombinant human SOD1/Superoxide Dismutase, aa 2-154.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008]
- Alternative Names
- Superoxide Dismutase 1; Superoxide Dismutase 1, Soluble; EC 1.15.1.1; HSod1; Amyotrophic Lateral Sclerosis 1 (Adult); Epididymis Secretory Protein Li 44; Superoxide Dismutase, Cystolic; Superoxide Dismutase [Cu-Zn]; Cu/Zn Superoxide Dismutase;
- Gene ID
- 6647
- UniProt ID
- P00441
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Related Diseases
Amyotrophic Lateral Sclerosis
Downloadable Resources
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Datasheet
MSDS
COA
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