CytoStream™ Rabbit Anti-SOD1 Recombinant Antibody (VS13-YC1092)
CAT#: VS13-YC1092
The CytoStream™ Rabbit Anti-SOD1 Recombinant Antibody is specific for human, mouse and rat SOD1. SOD1 encodes a cytoplasmic enzyme destroying superoxide radicals, with mutations linked to familial amyotrophic lateral sclerosis. The anti-SOD1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in glial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8670/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/30_D2_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/29_D2_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/31_D2_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/4148_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/4148_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/4148_A_8_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/4148_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/4148_A_4_6.jpg
Normal Tissue
Figure 10 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/4148_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000142168-SOD1
❮
❯
Specifications
- Immunogen
- Recombinant human superoxide dismutase 1, aa 105-154.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat SOD1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, ICC, IF, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000-1/2000
Immunofluorescence (IF-cell): 1/50-1/200
Immunofluorescence (IF-tissue): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Superoxide Dismutase 1; Superoxide Dismutase 1, Soluble; EC 1.15.1.1; HSod1; Amyotrophic Lateral Sclerosis 1 (Adult); Epididymis Secretory Protein Li 44; Superoxide Dismutase, Cystolic; Superoxide Dismutase [Cu-Zn]; Cu/Zn Superoxide Dismutase;
- Gene ID
- 6647
- UniProt ID
- P00441
- Long Name
- Superoxide Dismutase 1
- Cellular Localization
- Cytoplasm
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Related Diseases
Amyotrophic Lateral Sclerosis
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "SOD1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1320z | Mouse Anti-SOD1 Recombinant Antibody (clone 22C6) | ELISA, FC, ICC, IF, WB | Mouse IgG1 |
| VS3-FY1370 | Recombinant Rabbit Anti-SOD1 Antibody (clone R04-4C6) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| VS3-FY1371 | Recombinant Rabbit Anti-SOD1 Antibody (clone R07-1G6) | WB, IHC-P | Rabbit IgG |
| VS3-FY1372 | Recombinant Rabbit Anti-SOD1 Antibody (clone R08-5A5) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| VS7-0425-WR905 | Mouse Anti-SOD1 Monoclonal Antibody (VS7-0425-WR905) | WB, IF, ICC, FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-465CT | Anti-Human SOD1 Single Domain Antibody (TAB-465CT), Research Grade Biosimilar | ELISA, WB, IHC, IP | Single domain antibody |
| MD-ZBF-YC020 | Camelid Anti-SOD1 Recombinant Single Domain Antibody (MD-ZBF-YC020) | WB, IP, IHC, ELISA | Camelid VHH |
| MD-ZBF-YC021 | Camelid Anti-SOD1 Recombinant Single Domain Antibody (MD-ZBF-YC021) | WB, IP, IHC, ELISA | Camelid VHH |
| MD-ZBF-YC022 | Camelid Anti-SOD1 Recombinant Single Domain Antibody (MD-ZBF-YC022) | WB, IP, IHC, ELISA | Camelid VHH |
| MD-ZBF-YC023 | Camelid Anti-SOD1 Recombinant Single Domain Antibody (MD-ZBF-YC023) | WB, IP, IHC, ELISA | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-466CT | Mouse Anti-SOD1 Recombinant Antibody (TAB-466CT) | WB | Mouse IgG |
| TAB-467CT | Mouse Anti-SOD1 Recombinant Antibody (TAB-467CT) | WB, IF | Mouse IgG |
| TAB-468CT | Mouse Anti-SOD1 Recombinant Antibody (TAB-468CT) | ELISA, WB, BL, IHC | Mouse IgG |
| TAB-466CT-S(P) | Mouse Anti-SOD1 Recombinant Antibody; scFv Fragment (TAB-466CT-S(P)) | ELISA, WB, BL, IHC | Mouse scFv |
| TAB-467CT-S(P) | Mouse Anti-SOD1 Recombinant Antibody; scFv Fragment (TAB-467CT-S(P)) | ELISA, WB, BL, IHC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-469CT | Human Anti-SOD1 Recombinant Antibody (TAB-469CT) | ELISA | Human antibody |
| TAB-470CT | Human Anti-SOD1 Recombinant Antibody (TAB-470CT) | ELISA | Human antibody |
| TAB-471CT | Human Anti-SOD1 Recombinant Antibody (TAB-471CT) | ELISA | Human antibody |
| TAB-472CT | Human Anti-SOD1 Recombinant Antibody (TAB-472CT) | ELISA | Human antibody |
| TAB-473CT | Human Anti-SOD1 Recombinant Antibody (TAB-473CT) | IF, ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3369 | Hi-Affi™ Recombinant Rabbit Anti-SOD1 Monoclonal Antibody (DS3369AB) | ICC, IF, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY245 | AbPlus™ Anti-SOD1 Magnetic Beads (VS-0424-XY245) | IP, Protein Purification | |
| VS-0724-YC1461 | AbPlus™ Anti-SOD1 Magnetic Beads (VS-0724-YC1461) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2141 | Anti-SOD1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6803 | Anti-Mouse SOD1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6802 | Anti-Human SOD1 Immunohistochemistry Kit | IHC |
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